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L1210

L1210

Catalogue No.

87092804

Cell Line Name

L1210

Cell Line Description

Established from a tumour induced in an 8 month old female 234 subline 212 of a DBA mouse. Orginally subcultured in DBA/2 mice it was adapted to suspension culture. The line has been extensively used in cytotoxicity assays. Develops massive tumours in nude mice after a latent period of 7-10 days

Characteristics

Tissue of Origin

Blood

Karyotype

2n=40, diploid

Disease

Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 5x10,000 to 8x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

Fischer's medium + 2mM Glutamine + 10% Horse Serum (HS).

Growth Mode

Suspension

Additional Info

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Nat Cancer Inst 1949; 10:179; J Nat Cancer Inst 1966;36:405

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: L1210 (ECACC 87092804).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.