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RIN-5F

RIN-5F

Catalogue No.

95090402

Cell Line Name

RIN-5F

Cell Line Description

RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (ECACC catalogue no. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (ECACC catalogue no. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.

Characteristics

Products

Insulin, L-dopa-decarboxylase

Tissue of Origin

Pancreas

Karyotype

Not specified

Applications

Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin

Disease

Islet Cell Tumour

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Cells grow in small islands and do not form a confluent monolayer. Split sub-confluent cultures (40-50%) seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA.

 

Recommended growth conditions: 5% CO2, 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Natl Acad Sci USA 1977;74:628; ibid 1980;77:3519; Diabetes 1982;31:521; Endocrinology 1983;112:1070; Proc Soc Exp Biol Med 1983;175:35

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: RIN-5F (ECACC 95090402).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.