Fundamentals of Enzymology
Now in a new edition, this book provides an up-to-date account of the field of enzymology. it describes the action of enzymes in systems of increasing complexity, and shows how these properties can be best utilized, both clinically and technically. The reader will find in-depth coverage purified enzymes, including full treatment of purification methods, structure, kinetics, mechanism of action, and the control of enzyme activity. The behavior of enzymes in more complex systems is discussed, and the intact cell is thoroughy examined. Fundamentals of Enzymology also provides complete information on enzyme turnover in vivo, clinical aspects of enzymes, and enzyme technology. This new edition pays close attention to new applications of recombinant-DNA technology, and includes the latest information on the production of enzymes, the determination of amin-acid sequences, and the analysis of enzyme structure and function using site-directed mutagenesis.
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Common terms and phrases
6-phosphate adenosinetriphosphatase adenylate affinity amino acids aminotransferase arginine aspartate binding Biochemistry biol bonds carbamoyl phosphate catalysed catalytic activity cell cent centrifugation changes Chapter Chem chromatography chymotrypsin coli concentration containing covalent cytochrome cytosol D-glucose degradation elution enzyme activity enzyme kinetics enzyme-catalysed reactions Enzymes 3rd edn equilibrium example fatty-acid fraction fragments fructose-bisphosphatase fructose-bisphosphate aldolase glucose glutamate glycogen H₂O hexokinase hydrolysis inactivation inhibition inhibitor interactions intracellular involved isoenzymes isolated isomerase kinase kinetics lactate dehydrogenase ligand liver malate malate dehydrogenase mechanism membrane metabolic metabolites Methods Enzymol mitochondria mmol mmol dm³ molecules multienzyme protein muscle NAD+ NADH nucleotides nucleotidyltransferase occur ornithine orthophosphate oxaloacetate oxidase pathway peptide phosphatase phosphorylase polypeptide polypeptide chain procedure proteases purification pyruvate dehydrogenase reaction catalysed reference Section sequence serum side-chain specificity step structure studies subcellular substrate subunits synthase synthesis synthetase tissue Trends Biochem trypsin tryptophan Vmax X-ray