Principles of Fluorescence SpectroscopyThe third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine. |
Contents
Introduction to Fluorescence
|
1 |
Solvent and Environmental Effects
|
6 |
Energy Transfer
|
13 |
14
|
14 |
TimeResolved Protein Fluorescence
|
17 |
Novel Fluorophores
|
20 |
10
|
33 |
2
|
46 |
TimeResolved Energy Transfer
|
478 |
Energy Transfer to Multiple Acceptors
|
507 |
Protein Fluorescence
|
529 |
9
|
535 |
Quenching
|
547 |
41
|
552 |
280
|
577 |
Anisotropy Decay of Ribonuclease
|
585 |
BeerLambert
|
58 |
1
|
60 |
7
|
64 |
5
|
66 |
10
|
81 |
144
|
85 |
TimeDomain Lifetime Measurements
|
91 |
3
|
99 |
6
|
105 |
DNA Sequencing
|
123 |
7
|
161 |
4
|
183 |
Decay Rates
|
222 |
Overview of ExcitedState Processes
|
237 |
9
|
243 |
33
|
245 |
Quenching of Fluorescence
|
277 |
10
|
281 |
34
|
297 |
Applications of Quenching to Molecular
|
310 |
2
|
315 |
257
|
326 |
4
|
328 |
Mechanisms and Dynamics
|
332 |
37
|
335 |
Effects
|
343 |
Fluorescence Anisotropy
|
353 |
References
|
378 |
364
|
393 |
Advanced Anisotropy Concepts
|
413 |
7
|
424 |
40
|
437 |
44
|
598 |
315
|
605 |
Multiphoton Excitation and Microscopy
|
608 |
Fluorescence Sensing
|
623 |
281
|
660 |
316
|
664 |
317
|
715 |
Quenching by Gold
|
723 |
45
|
726 |
FluorescenceLifetime Imaging Microscopy
|
741 |
6
|
744 |
SingleMolecule Detection
|
757 |
46
|
768 |
318
|
795 |
Fluorescence Correlation Spectroscopy
|
797 |
13
|
803 |
Detection of Conformational Dynamics
|
820 |
49
|
851 |
52
|
858 |
Corrected Emission Spectra
|
873 |
327
|
877 |
889 | |
923 | |
925 | |
929 | |
933 | |
934 | |
942 | |
943 | |
948 | |
949 | |