An Introduction to Genetic Engineering

Front Cover
Cambridge University Press, May 29, 2008 - Science
In this third edition of his popular undergraduate-level textbook, Des Nicholl recognises that a sound grasp of basic principles is vital in any introduction to genetic engineering. Therefore, the book retains its focus on the fundamental principles used in gene manipulation. It is divided into three sections: Part I provides an introduction to the relevant basic molecular biology; Part II, the methods used to manipulate genes; and Part III, applications of the technology. There is a new chapter devoted to the emerging importance of bioinformatics as a distinct discipline. Other additional features include text boxes, which highlight important aspects of topics discussed, and chapter summaries, which include aims and learning outcomes. These, along with key word listings, concept maps and a glossary, will enable students to tailor their study to suit their own learning styles and ultimately gain a firm grasp of a subject that students traditionally find difficult.
 

Contents

2Laying the foundations
5
The basis of genetic engineering
11
x
17
viii
23
Working with nucleic acids
32
vi
40
The tools of the trade
50
The methodology of gene manipulation
61
Bioinformatics
152
Genetic engineering in action
169
Genetic engineering and biotechnology
202
Medical and forensic applications of gene
226
Transgenic plants and animals
256
The other sort of cloning
278
Brave new world or genetic nightmare?
291
Suggestions for further reading
297

Cloning strategies
90
The polymerase chain reaction
116
4Processing of PCR products
128
Glossary
303
Index
327
Copyright

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About the author (2008)

Des Nicholl is a Senior Lecturer in Biological Sciences at the University of the West of Scotland, Paisley, Scotland, UK.

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