Abstract
Honey is a completely natural product. Although most commercially distributed honey has been subjected to some degree of physical processing, raw honey can be used straight from the comb as taken from the beehive. The distinction of honey as a pure natural product is maintained by food regulations worldwide: anything sold as honey has to have been produced by bees from the secretions of plants, and be free from any additives and contaminants. Nevertheless, the fraudulent practice of selling adulterated or simulated honey is widespread and has gone on since ancient times. To understand the challenge presented in detecting such fraud it is necessary to be aware of the complexity of honey, a natural product with a very varied nature resulting from the wide variability of its sources.
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References
Adams, R.J. and Smith, M.V. (1981) Seasonal pollen analysis of nectar from the hive and of extracted honey. J. Apicultural Res. 20(4), 234–48.
Ali, A.T.M.M., Al-Humayyd, M.S. and Madan, B.R. (1990) Natural honey prevents indomethacin- and ethanol-induced gastric lesions in rats. Saudi Med. J. 11, 275–79.
Al-Somai, N., Coley, K.E., Molan, P.C. et al. (1994) Susceptibility ofHelicobacter pylori to the antibacterial activity of manuka honey. J. Royal Soc. Med. 87(1), 9–12.
Allen, K.L., Molan, P.C. and Reid, G.M. (1991) A survey of the antibacterial activity of some New Zealand honeys. J. Pharm. Pharmacol. 43(12), 817–22.
Baker, H.G. and Baker, I. (1977) Intraspecific constancy of floral nectar amino acid complements. Botanical Gazette 138(2), 183–191.
Baker, H.G. and Baker, I. (1983) A brief historical review of the chemistry of floral nectar. In: The Biology of Nectaries (Eds B. Bentley and T. Elias). Columbia University Press, New York, pp. 126–52.
Baker, H.G., Opler, P.A. and Baker, I. (1978) A comparison of the amino acid complements of floral and extrafloral nectars. Botanical Gazette 139, 322–32.
Bicchi, C., Belliardo, F. and Frattini, C. (1983) Identification of the volatile components of some Piedmontese honeys. J. Apicultural Res. 22(2), 130–36.
Blank, I., Fischer, K.-H. and Grosch, W. (1989) Intensive neutral odourants of linden honey. Zeitschrift für Lebensmittel-Untersuchung und-Forschung 189, 426–33.
Bogdanov, S. and Baumann, E. (1988) Bestimmung von Honigzuckern mit HPLC. Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene 79, 198–206.
Bonaga, G. and Giumanini, A.G. (1986) The volatile fraction of chestnut honey. J. Apicultural Res. 25(2), 113–20.
Bosi, G. and Battaglini, M. (1978) Gas chromatographic analysis of free and protein amino acids in some unifloral honeys.J. Apicultural Res. 17(3), 152–66.
Bricout, J. (1973) Control of authenticity of fruit juices by isotopic analysis. J. Assoc. Official Analytical Chem. 56(3), 739–42.
Brookes, S.T., Barrie, A. and Davies, J.E. (1991) A rapid 13C/12C test for determination of corn syrups in honey. J. Assoc. Official Analytical Chem. 74(4), 627–29.
Burroughs, L.F. and Otlet, R.L. (1986) The proline contents and stable carbon isotope ratio of genuine United Kingdom honey. J. Assoc. Public Analysis 24, 91–93.
Chataway, H.D. (1932) Determination of moisture content in honey. Canad. J. Res. 6, 532–47.
Church, J. (1954) Honey as a source of the anti-stiffness factor. Fed. Proc. Am. Physiol. Soc. 13(1), 26.
Crane, E. (1975) The flowers honey comes from. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 3–76.
Crane, E., Walker, W. and Day, R. (1984) Directory of Important World Honey Sources, International Bee Research Association, London.
Cremer, E. and Riedmann, M. (1964) Identifizierung von gaschromatographisch getrennten Aromastoffen in Honigen. Zietschrift für Naturforschung B 19(1), 76–77.
Cremer, E. and Riedmann, M. (1965) Gaschromatographisch Untersuchungen zur Frage des Honigaromas. Monatshefte für Chemie 96(2), 364–68.
Croft, L. (1983) UK beekeeping under threat. In: The Sunday Times, London, 20 February, p. 22.
Curti, R. and Riganti, V. (1966) Richerche sigli aminoacidi del miele. Rassegna Chimica 18(6), 278–82.
Davies, A.M.C. (1975) Amino acid analysis of honeys from eleven countries. J. Apicultural Res. 14(1), 29–39.
Davies, A.M.C. (1976) The application of amino acid analysis to the determination of geographical origin of honey. J. Food Technol. 11, 515–23.
Dommee, B., Assouad, M.W. and Vadeyron, G. (1978) Natural selection and gynodioecy in Thymus vulgaris. Botanical J. Linnean Soc. 77, 17–28.
Doner, L.W. (1977) The sugars of honey—a review. J. Sci. Food Agric. 28, 443–56.
Doner, L.W. and White, J.W. (1977) Carbon-13/carbon-12 ratio is relatively uniform among honeys. Science 197 891–92.
Doner, L.W., Kushnir, I. and White, J.W. (1979a) Assuring the quality of honey. Is it honey or is is syrup? Analyt. Chem. 51(2), 224A-232A.
Doner, L.W., White, J.W. and Phillips, J.G. (1979b) Gas-liquid chromatographic test for honey adulteration by high fructose corn sirup.J. Assoc. Official Analytical Chem. 62(1), 186–89.
Doner, L.W., Brause, A.R. and Petrus, D.R. (1992) δ18O measurements in water for detection of sugar beet-derived syrups in frozen concentrated orange juice: collaborative study. J. AOAC Internat. 75(6), 1107–11.
Dörrscheidt, W. and Friedrich, K. (1962) Trennung von Aromastoffen des Honigs mit hilfe der Gas-Chromatographie. J. Chromatogr. 7(1), 13–18.
Duisberg, H. and Hadorn, H. (1966) Weiche Anforderungen sind an Handelshonige zu stellen? Vorschläge auf Grund der statistichen Auswertung von ca. 1600 Honig-Analysen. Mitteilungen aus dem Gebiete der Lebensmitteluntersuchung und Hygiene 57(5), 386–407.
Durkee, L.T. (1983) The ultrastructure of floral and extrafloral nectaries. In: The Biology of Nectaries (Eds B. Bentley and T. Elias). Columbia University Press, New York, pp. 1–29.
Dustmann, J.H. (1971) Über die Katalaseaktivität in Bienenhonig aus der Tracht deer Heidekrautgewächse (Ericaceae). Zeitschrift für Lebensmittel-Untersuchung und-Forschung 145, 294–95.
Dyce, E.J. (1975) Producing finely granulated or creamed honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 293–306.
Editorial (1981) American Bee Journal 121(12), 860.
Fasler, A. (1975) Honey standards legislation. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 329–54.
Fossel, A. (1968) Pollenersatzmittel im mikroskopischen Befund von Frühtrachthonigen. Zeitschrift für Bienenforschung 9(5), 206–11.
Gary, N.E. (1992) Activities and behaviour of honey bees. In: The Hive and the Honey Bee, revised edn. (Ed. J.M. Graham). Dadant & Sons, Hamilton, Illinois, pp. 269–372.
Genier, G. (1966) Le pollen des Ericaceae dans le miels français. Annales de l’Abeille (Paris) 9(4), 271–321.
Gilbert, J., Shepherd, M.J., Wallwork, M. A. et al. (1981) Determination of the geographical origin of honeys by multivariate analysis of gas chromatographic data on their free amino acid content. J. Apicultural Res . 20(2), 125–35.
Graddon, A.D., Morison, J.D. and Smith, J.F. (1979) Volatile constituents of some unifloral Australian honeys. J. Agricultural Food Chem. 27(4), 832–37.
Graham, J. (1979) Honey adulteration is not a new problem. Am. Bee J. 119(3), 228–29.
Gray, R. (1952) Composition of honeydew excreted by pinapple mealybugs. Science 115, 129–33.
Hagedorn, J.L. (1992) Differentiation of natural and synthetic benzaldehydes by 2H nuclear magnetic resonance. J. Agricultural Food Chem. 40(4), 634–37.
Häusler, M. and Montag, A. (1989) Isolation, identification and quantitative determination of the isoprenoid (S)-(+)-dehydrovomifoliol in honey. Zeitschrift für Lebensmittel-Untersuchung und-Forschung 189, 113–15.
Haydak, M.H., Crane, E., Duisberg, H. et al. (1975) Biological properties of honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 258–66.
Howells, V.W. (1969) Some reflections on the pollen analysis of honey. J. Assoc. Public Analysts 7, 88–93.
Jacobs, M.B. (1955) Flavouring with honey.Am. Perfumer Essential Oil Rev. 66(1), 46–47.
Kandil, A., El-Banby, M., Abdel-Wahed, K. et al. (1987) Curative properties of true floral and false non-floral honeys on induced gastric ulcer. J. Drug Res. (Cairo) 17, 103–106.
Kirkwood, K.C., Mitchell, T.J. and Smith, D. (1960) An examination of the occurrence of honeydew in honey. Analyst, London 85(1011), 412–16.
Kirkwood, K.C., Mitchell, T.J. and Ross, I.C. (1961) An examination of the occurrence of honeydew in honey. Part II. Analyst, London 86(1020), 164–65.
Komamine, A. (1960) Amino acids in honey.Acta Chemica Fennica B33, 185–87.
Kushnir, I. (1979) Sensitive thin-layer chromatographic detection of high-fructose corn syrup and other adulterants in honey. J. Assoc. Official Analytical Chem. 62(4), 917–20.
Lipp, J., Ziegler, H. and Conrady, E. (1988) Detection of high fructose and other syrups in honey using high-pressure liquid chromatography. Zeitschrift für Lebensmittel-Untersuchung und-Forschung 187(4), 334–38.
Lothrop, R.E. (1932) Specific test for orange honey. Indust. Engineering Chem.—Analytical Edn. 4 395–96.
Louveaux, J., Maurizio, A. and Vorwohl, G. (1978) Methods of melissopalynology. Bee World 59(4), 139–57.
Low, N.H., Brisbane, T., Bigam, G. et al. (1988) Carbon-13 nuclear magnetic resonance for the qualitative and quantitative analysis of structurally similar disaccharides. J. Agric. Food Chem. 36(5), 953–57.
Marek, M., Bacilek, J. and Jary, J. (1980) Polarographic determination of the adulteration of honey with invert sugar. J. Apicultural Res. 19(4), 255–60.
Matheson, A. (1993) Honey research brings commercial success. Bee World 74(3), 145–6.
Maurizio, A. (1962) From the raw material to the finished product: honey. Bee World 43, 66–81.
Maurizio, A. (1973) The heather honeys of Europe.Bee World 54(3), 111–16.
Maurizio, A. (1975a) How bees make honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 77–105.
Maurizio, A. (1975b) Microscopy of honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 240–57.
Maurizio, A. and Louveaux, J. (1964) Pollens des plantes melliferes d’Europe.Pollen et Spores 3, 219–346.
Moar, N.T. (1985) Pollen analysis of New Zealand honey. NZJ. Agric. Res. 28, 39–70.
Molan, P.C. (1992) The antibacterial activity of honey. 1. The nature of the antibacterial activity. Bee World 73(1), 5–28.
Molan, P.C. (1992b) The antibacterial activity of honey. 2. Variation in the potency of the antibacterial activity. Bee World 73(2), 59–76.
Moore, P.D. and Webb, J.A. (1978) An Illustrated Guide to Pollen Analysis, Hodder and Stoughton, London.
Morton, I.D. and Sharples, E. (1959) Process of preparing a honey flavour. U.S. Patent No. 2 916 382.
Nixon, H.L. and Ribbands, C.R. (1952) Food transmission within the honeybee community. Proc. Royal Soc. London, Series B 140, 43–50.
Percival, M.S. (1961) Types of nectar in angiosperms. The New Phytologist 60, 235–81.
Perez-Arquillué, C., Conchello, P., Arino, A. et al. (1994) Quality evaluation of Spanish rosemary (Rosmarinus officinalis) honey. Food Chem. 51(2), 207–10.
Riley, D. and Young, A. (1966) World Vegetation, Cambridge University Press, London.
Rinderer, T.E. and Baxter, J.R. (1980) Honeybee Apis mellifera hoarding of high-fructose corn syrup and cane sugar syrup. Am. Bee J. 120(12), 817–18.
Rodgers, P.E.W. (1975) Honey quality control. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 314–25.
Roßmann, A., Lüllmann, C. and Schmidt, H.-L. (1992) Massenspektometrische Kohlenstoff-und Wasserstoff-Isotopen-Verhältnismessung zur Authentizitätsprüfung bei Honigen. Zeitschrift für Lebensmittel-Untersuchung und-Forschung 195(4), 307–11.
Rubenstein, R.M. (1977) Honey adulteration testing.Am. Bee J. 117(12), 736–37.
Sawyer, R.W. (1975) Melissopalynology in the determination of the geographical and floral origin of honey. J. Assoc. Public Analysts 13, 64–71.
Sawyer, R.W. (1988) Honey Identification, Cardiff Academic Press, Cardiff, UK.
Schade, J.E., Marsh, G.L. and Eckert, J.E. (1958) Diastase activity and hydroxymethylfurfural in honey and their usefulness in detecting heat alteration. Food Res. 23, 446–63.
Schmidt, H.-L., Butzenlechner, M., Rossmann, A. et al. (1993) Inter- and intramolecular isotope correlations in organic compounds as a criterion for authenticity identification and origin assignment. Zeitschrift für Lebensmittel-Untersuchung und-Foreschung 196(2), 105–10.
Shuel, R.W. (1992) The production of nectar and pollen. In: The Hive and the Honey Bee, revised edn. (Ed. J.M. Graham). Dadant & Sons, Hamilton, Illinois, pp. 401–36.
Siddiqui, I.R. (1970) The sugars of honey.Adv. Carbohydrate Chem. Biochem. 25, 285–309.
Sidorciuc, D. and Popescu, N. (1972) [Man-made honey]. Rumanian Patent No. 53959.
Simpson, J., Moxley, E. and Greenwood, S.P. (1974) Can honey from sugar-fed bees be distinguished from natural honey by its flavour? Bee World 55(1), 10–14.
Solov’eva and Bazarova, V. I. (1963) The content of free amino acids in some unifloral honeys [in Russian]. Voprosy Pitaniya 22, 69–70.
Speer, K. and Montag, A. (1986) Verteilung freier Aminosären in Honigen unter besonderer Berücksichtigung der deutschen un der französischen Heidehonige. Deutsche Lebensmittel-Rundschau 82(8), 248–53.
Sporns, P. (1981) High-pressure liquid chromatographic determination of phenol in honey. J. Assoc. Official Analytical Chem. 64(2), 337–39.
Stockmarr, J. (1971) Tablets with spores used in absolute analysis. Pollen et Spores 13, 615–21.
Swallow, K.W. and Low, N.H. (1994) Determination of honey authenticity by anionexchange liquid-chromatography. J. AOAC Internat. 77(3) 695–702.
Takeba, K., Matsumoto, M., Shida, Y. et al. (1990) Determination of phenol in honey by liquid chromatography with amperometric detection. J. Assoc. Official Analytical Chem. 73(4), 602–04.
Tan, S.T. (1989) A chemical investigation of some New Zealand honeys. DPhil. Thesis, University of Waikato, Hamilton, New Zealand.
Tan, S.T., Holland, P.T., Wilkins, A.L. et al. (1988) Extractives from New Zealand honeys. 1. White clover, manuka and kanuka unifloral honeys. J. Agric. Food Chem. 36(3), 453–60.
Tan, S.T., Wilkins, A.L., Holland, P.T. and McGhie, T.K. (1989a) Extractives from New Zealand unifloral honeys. 2. Degraded carotenoids and other substances from heather honey. J. Agric. Food Chem. 37(5), 1217–21.
Tan, S.T., Wilkins, A.L., Holland, P.T. et al. (1990) Extractives from New Zealand honeys. 3. Unifloral thyme and willow honey. J. Agric. Food Chem. 38(9), 1833–38.
Tan, S.T., Wilkins, A.L., Molan, P.C. et al. (1989b) A chemical approach to the determination of the floral sources of New Zealand honeys. J. Apicultural Res. 28(4), 212–22.
Townsend, G.F. (1975) Processing and storing liquid honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 269–92.
Trumpeter, J.N. (1981) Clarifying some of the myths and confusion about HFCS, sugar and honey. Am. Bee J. 121(12), 857–63.
Verordmung über den Verkehn mit Lebensmittein (1971) Art. 217/3.
Vorwohl, G. (1964) Die Beziehungen zwischen der elektrischen Leitfähigkeit der Honige und ihrer trachtmässigen Herkunft. Annales de l’Abeille 7(4), 301–9.
Walsh, R.S. (1960) Anti-granulation method for honey packers. NZJ. Agric. 10(3), 229–31.
White, J.W. (1966) Methyl anthranilatc content of citrus honey. J. Food Sci. 31(1), 102–04.
White, J.W. (1975a) Composition of honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 157–206.
White, J.W. (1975b) Physical characteristics of honey. In:Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 207–39.
White, J.W. (1979a) Spectrophotometric method for hydroxymethylfurfural in honey. J. Assoc. Official Analytical Chem. 62(3), 509–14.
White, J.W. (1979b) Methods for determining carbohydrates, hydroxymethylfurfural, and proline in honey: collaborative study. J. Assoc. Official Analytical Chem. 62(3), 515–26.
White, J.W. (1980a) Hydroxmethylfurfural content of honey as an indicator of its adulteration with invert sugars. Bee World 61(1), 29–37.
White, J.W. (1980b) Detection of honey adulteration by carbohydrate analysis. J. Assoc. Official Analytical Chem. 63(1), 11–18.
White, J.W. (1980c) High-fructose corn syrup adulteration of honey: confirmatory testing required with certain isotope ratio values. J. Assoc. Official Analytical Chem. 63(5), 1168.
White, J.W. (1981) The honey industry council fights adulteration. Am. Bee J. 121(6), 452–53.
White, J.W. (1987) Wiley led the way: a century of federal honey research. J. Assoc. Official Analytical Chem. 70(2) 181–89.
White, J.W. (1992a) Honey. In: The Hive and the Honey Bee, revised edn. (Ed. J.M. Graham), Dadant & Sons, Hamilton, Illinois, pp. 869–925.
White, J.W. (1992b) Internal standard stable carbon isotope ratio method for determination of C-4 plant sugars in honey: collaborative study, and evaluation of improved protein preparation procedure. J. AOAC Internat. 75(3), 543–48.
White J.W. (1994) The role of HMF and diastase assays in honey quality evaluation. Bee World 75(3), 104–17.
White, J.W. and Doner, L.W. (1978a) The 13C/12C ratio in honey.J. Apicultural Res. 17(2), 94–99.
White, J.W. and Doner, L.W. (1978b) Mass spectrometric detection of high-fructose corn sirup in honey by use of 13C/12C ratio: collaborative study. J. Assoc. Official Analytical Chem. 61(3), 746–50.
White, J.W. and Rudyk, O.N. (1978a) The proline content of United States honeys.J. Apicultural Res. 17(2), 89–93.
White, J.W. and Rudyj, O.N. (1978b) The protein content of honey. J. Apicultural Res. 17(4), 234–38.
White, J.W. and Siciliano, J. (1980) Hydroxmethylfurfural and honey adulteration. J. Assoc. Official Analytical Chem. 63(1), 7–10.
White, J.W. and Winters, K. (1989) Honey protein as internal standard for stable carbon isotope ratio detection of adulteration of honey.J. Assoc. Official Analytical Chem. 72(6), 907–11.
White, J.W., Meloy, R.W., Probst, J.L.et al. (1986) Detection of beet sugar adulteration of honey. J. Assoc. Official Analytical Chem. 69(4), 652–54.
White, J.W., Riethof, M.L. and Kushnir, I. (1961) Composition of honey, VI. The effect of storage of carbohydrates, and diastase content. J. Food Sci. 26(1) 63–71.
White, J.W., Riethof, M.L., Subers, M.H. et al. (1962) Composition of American honeys. In: US Department of Agriculture Technical Bulletin 1261.
White, J.W., Subers, M.H. and Schepartz, A.I. (1963) The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochimica et Biophysica Acta 73, 57–70.
White, J.W., Bryant, V.M. and Jones, J.G. (1991) Adulteration testing of Southwestern Desert honeys. Am. Bee J. 131(2), 123–26, 135.
Wilkins, A.L., Lu, Y. and Molan, P.C. (1993a) Extractable organic substances from New Zealand unifloral manuka (Leptrospermum scoparium) honeys. J. Apicultural Res. 32(1), 3–9.
Wilkins, A.L., Lu, Y. and Tan, S.T. (1993b) Extractives from New Zealand honeys. 4. Linalool derivatives and other components from nodding thistle (Carduus nutans) honey. J. Agric. Food Chem. 41(6), 873–78.
Willix, D.J., Molan, P.C. and Harfoot, C.J. (1992) A comparison of the sensitivity of wound-infecting species of bacteria to the antibacterial activity of manuka honey and other honey. J. Appl. Bacteriol. 73, 388–94.
Willson, R.B. (1975) World trading in honey. In: Honey: A Comprehensive Survey (Ed. E. Crane). Heinemann, London, pp. 355–77.
Winkler, O. (1955) Beitrag zum Nachweis und zur Bestimmung von Oxymethylfufural in Honig und Kunsthonig. Zeitschrift für Lebensmittel-Untersuchung und-Forschung 102(3), 161–67.
Wootton, M., and Ryall, L. (1985) A comparison of Codex Alimentarius Commission and HPLC methods for 5-hydroxymethyl-2-furaldehyde determination in honey.J. Apicultural Res. 24(2), 120–24.
Wootton, M., Edwards, R.A. and Faraji-Haremi, R. (1978) Effect of accelerated storage conditions on the chemical composition and properties of Australian honeys 3. Changes in volatile components. J. Apicultural Res. 17(3), 167–72.
Wykes, G.R. (1952) An investigation of the sugars present in the nectar of flowers of various species. The New Phytologist 51, 210–15.
Ziegler, H., Stichler, W. Maurizio, A. et al. (1978) Die Verwendung stabiler Isotope zur Charakterisierung von Honigen, ihrer Herkunft und ihrer Verfälschung. Apidologie 8(4), 337–47.
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Molan, P.C. (1996). Authenticity of honey. In: Ashurst, P.R., Dennis, M.J. (eds) Food Authentication. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1119-5_8
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