Cell
Volume 65, Issue 3, 3 May 1991, Pages 417-427
Journal home page for Cell

Late endosomes derive from early endosomes by maturation

https://doi.org/10.1016/0092-8674(91)90459-C Get rights and content

Abstract

Endocytosed proteins destined for degradation in lysosomes are targeted mainly to early endosomes following uptake. Late endosomes are the major site for entry of newly synthesized lysosomal hydrolases via the cation-independent mannose 6-phosphate receptor into the degradative pathway. No consensus exists as to the mechanism of transport from early to late endosomes. We used asialoorosomucoid and transferrin to label selected parts of the degradative and receptor-recycling pathways, respectively, in the human hepatoma cell line HepG2. Intracellular mixing of sequentially endocytosed 125I- and HRP-labeled ligands was monitored by using 3,3′-diaminobenzidine-mediated density perturbation. The entire endocytic pathway of asialoorosomucoid, except for the lysosomes, remained fully accessible to subsequently endocytosed transferrin conjugated to HRP with unchanged kinetics. These results together with immunoelectron microscopic data support a model in which early endosomes gradually mature into late endosomes.

References (56)

  • M. Marsh et al.

    Adsorptive endocytosis of Semliki Forest virus

    J. Mol. Biol.

    (1980)
  • E. Regoeczi et al.

    Diacytosis of human asialotransferrin type 3 in the rat liver is due to the sequential engagement of two receptors

    Exp. Cell Res.

    (1985)
  • A.L. Schwartz et al.

    Biosynthesis of the human asialoglycoprotein receptor

    J. Biol. Chem.

    (1983)
  • A.L. Schwartz et al.

    Difficulties in the quantitation of asialoglycoprotein receptors on the rat hepatocyte

    J. Biol. Chem.

    (1980)
  • A.L. Schwartz et al.

    Kinetics of internalization and recycling of the asialoglycoprotein receptor in a hepatoma cell line

    J. Biol. Chem.

    (1982)
  • J.W. Slot et al.

    Localization of macromolecular components by application of the immunogold technique on cryosectioned bacteria

    Meth. Microbiol.

    (1988)
  • B. VanDeurs et al.

    The ways of endocytosis

    Int. Rev. Cytol.

    (1989)
  • D.A. Wall et al.

    The galactosespecific recognition system of mammalian liver: the route of ligand internalization in rat hepatocytes

    Cell

    (1980)
  • P.H. Weigel et al.

    Recycling of the hepatic asialoglycoprotein receptor in isolated rat hepatocytes: receptor-ligand complexes in an intracellular slowly dissociating pool return to the cell surface prior to dissociation

    J. Biol. Chem.

    (1984)
  • M.C. Willingham et al.

    The receptosome: an internal intermediate organelle of receptor-mediated endocytosis in cultured fibroblasts

    Cell

    (1980)
  • R.S. Ajioka et al.

    Intracellular pools of transferrin receptors result from constitutive internalization of unoccupied receptors

  • G. Ashwell et al.

    The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins

    Adv. Enzymol.

    (1974)
  • M. Benveniste et al.

    Characterization of internalization and endosome formation of epidermal growth factor in transfected NIH-3T3 cells by computerized image-intensified threedimensional fluorescence microscopy

    J. Cell Biol.

    (1989)
  • W.A. Braell

    Fusion between endocytic vesicles in a cell free system

  • K. Bridges et al.

    Fate of receptor and ligand during endocytosis of asialoglycoproteins by isolated hepatocytes

  • A. Dautry-Varsat et al.

    pH and the recycling of transferrin during receptor-mediated endocytosis

  • K.W. Dunn et al.

    Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome

    J. Cell Biol.

    (1989)
  • H.J. Geuze et al.

    Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections

    J. Cell Biol.

    (1981)
  • Cited by (268)

    • Iron homeostasis and organismal aging

      2021, Ageing Research Reviews
    View all citing articles on Scopus
    View full text