Abstract
THE formation of single, stable bimolecular lipid and proteolipid1 membranes up to 10 mm.2 in area has been accomplished routinely in 0.1 M saline solution by methods analogous to the formation of Hooke-Newton ‘secondary black’ in air soap films2–4. By forming such a membrane between two compartments filled with saline its transverse electrical properties can be measured, and controlled chemical investigations can be undertaken.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Folch, J., and Lees, M., J. Biol. Chem., 191, 807 (1951).
Hooke, R., Roy. Soc. Meeting, 1672, in The History of the Roy. Soc. of London, 3, 29 by Thomas Birch (A. Millar, London, 1757).
Newton, I., Opticks (1704), 214 and 233 (reprinted by Dover, New York, 1952).
Perrin, J., Ann. Phys., 10, 160 (1918).
Blinks, L. R., J. Gen. Physiol., 19, 633 (1936).
Finkelstein, A., Nature, 190, 1119 (1961).
Mueller, P., J. Gen. Physiol., 42, 137 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MUELLER, P., RUDIN, D., TI TIEN, H. et al. Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System. Nature 194, 979–980 (1962). https://doi.org/10.1038/194979a0
Issue Date:
DOI: https://doi.org/10.1038/194979a0
This article is cited by
Revealing the single-channel characteristics of OprD (OccAB1) porins from hospital strains of Acinetobacter baumannii
European Biophysics Journal (2023)
Challenges and opportunities in achieving the full potential of droplet interface bilayers
Nature Chemistry (2022)
Ion currents through Kir potassium channels are gated by anionic lipids
Nature Communications (2022)
Liquid–liquid phase separation as an organizing principle of intracellular space: overview of the evolution of the cell compartmentalization concept
Cellular and Molecular Life Sciences (2022)
3D Artificial Cell Membranes as Versatile Platforms for Biological Applications
BioChip Journal (2022)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
