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Phase–contrast X–ray computed tomography for observing biological soft tissues

An Erratum to this article was published on 01 May 1996

Abstract

Biological soft tissues are almost transparent to hard X rays and therefore cannot be investigated without enhancement with a contrast medium, such as iodine. On the other hand, phase–contrast X–ray imaging is sensitive to light elements1–8. This is because the X–ray phase shift cross section is almost a thousand times larger than the X–ray absorption cross section for light elements such as hydrogen, carbon, nitrogen and oxygen4,5. Hence, phase–contrast X–ray imaging is a promising technique for observing the structure inside biological soft tissues without the need for staining and without serious radiation exposure. We have devised a means of observing biological tissues in three dimensions using a novel X–ray computed tomography (CT) by modifying the phase–contrast technique. To generate appropriate CT input data, we used phase–mapping images obtained using an X–ray interferometer6 and computer analysis of interference patterns9. Now, we present a three–dimensional observation result of a nonstained sample of a cancerous rabbit liver, using a synchrotron X–ray source. Phase–contrast X–ray CT was able to clearly differentiate the cancer lesion from the normal tissue. Moreover, fine structures corresponding to cancerous degeneration and fibrous tissues were clearly depicted.

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References

  1. Bonse, U. & Hart, M., X-ray interferometer with long interfering beam paths. Appl. Phys. Lett. 1, 99–101 (1965).

    Article  Google Scholar 

  2. Ando, M. & Hosoya, S. An attempt at X-ray phase-contrast microscopy. Proc. 6th International Conference of X-ray Optics and Microanalysis (ed. Shinoda, G.; Kohra, K. and Ichinokawa, T.) 63–68 (University of Tokyo Press, 1972).

    Google Scholar 

  3. Hart, M. Ten years of X-ray interferometer. Proc. R. Soc. Land. A346, 1–22 (1975).

    Article  Google Scholar 

  4. Momose, A. & Fukuda, J. Phase-contrast radiographs of nonstained rat cerebellar specimen. Med. Phys. 22, 375–380 (1995).

    Article  CAS  Google Scholar 

  5. Takeda, T., Momose, A., Itai, Y., Wu, J. & Hirano, K. Phase-contrast imaging with synchrotron X-rays for cancer lesion. Acad. Radial. 2, 799–803 (1995).

    Article  CAS  Google Scholar 

  6. Bonse, U. & Hart, M., X-ray interferometer. Appl. Phys. Lett. 6, 155–156 (1965).

    Article  Google Scholar 

  7. Momose, A. Demonstration of phase-contrast X-ray computed tomography using an X-ray interferometer. Nucl. lustrum. Methods A352, 622–628 (1995).

    Article  Google Scholar 

  8. Momose, A., Takeda, T. & Itai, Y., X-ray computed tomography for observing biological specimens and organic materials. Rev. Sci. Instrum. 66, 1434–1436 (1995).

    Article  CAS  Google Scholar 

  9. Bruning, J.H. et al. Digital wavefront measuring interferometer for testing optical surfaces and lenses. Appl. Opt. 13, 2693–2703 (1974).

    Article  CAS  Google Scholar 

  10. McNulty, I. The future of X-ray holography. Nucl. Instrum. Methods A347, 170–176 (1994).

    Article  Google Scholar 

  11. Momose, A., Takeda, T. & Itai, Y. Contrast effect of blood on phase-contrast X-ray imaging. Acad. Radiol. 2, 883–887 (1995).

    Article  CAS  Google Scholar 

  12. Deslattes, R.D. & Henins, A. X-ray to visible wavelength ratios. Phys. Rev. Lett. 31, 972–975 (1973).

    Article  CAS  Google Scholar 

  13. Becker, P. et al. Absolute measurement of the (220) lattice plane spacing in a silicon crystal. Phys. Rev. Lett. 46, 1540–1543 (1981).

    Article  CAS  Google Scholar 

  14. Suzuki, Y. et al. X-ray sensing pickup tube. Rev. Sci. Instrum. 60, 2299–2302 (1989).

    Article  CAS  Google Scholar 

Download references

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Momose, A., Takeda, T., Itai, Y. et al. Phase–contrast X–ray computed tomography for observing biological soft tissues. Nat Med 2, 473–475 (1996). https://doi.org/10.1038/nm0496-473

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