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Neutron Diffraction Studies on the Location of Water in Lecithin Bilayer Model Membranes

January 1, 1975
72 (1) 376-380

Abstract

Lamellar neutron diffraction from oriented multilayers of hydrated dipalmitoyl lecithin was phased by isomorphous H2O-D2O exchange and swelling techniques. Bound water sites were located in the polar head group region of the bilayer profile. A 6-Å resolution structure based on the neutron scattering density profile is proposed for the bilayer. It is consistent with the electron density profile from x-ray diffraction.

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Published in

Go to Proceedings of the National Academy of Sciences
Go to Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences
Vol. 72 | No. 1
January 1975
PubMed: 16592215

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    Submission history

    Published online: January 1, 1975
    Published in issue: January 1975

    Keywords

    1. membrane structure
    2. bound water
    3. H2O-D2O exchange
    4. phospholipid bilayer

    Authors

    Affiliations

    G. Zaccai
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973
    J. K. Blasie
    Department of Biophysics and Physical Biochemistry, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pa. 19174
    B. P. Schoenborn
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973

    Notes

    Present address: Institut Laue-Langevin, B.P. 156 Centre de Tri, 38042 Grenoble Cedex, France.

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      Neutron Diffraction Studies on the Location of Water in Lecithin Bilayer Model Membranes
      Proceedings of the National Academy of Sciences
      • Vol. 72
      • No. 1

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