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DNA sequencing with chain-terminating inhibitors

December 1, 1977
74 (12) 5463-5467

Abstract

A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.

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Published in

Go to Proceedings of the National Academy of Sciences
Go to Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences
Vol. 74 | No. 12
December 1977
PubMed: 271968

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Submission history

Published online: December 1, 1977
Published in issue: December 1977

Keywords

  1. DNA polymerase
  2. nucleotide sequences
  3. bacteriophage ϕX174

Authors

Affiliations

F. Sanger
Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England
S. Nicklen
Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England
A. R. Coulson
Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England

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    DNA sequencing with chain-terminating inhibitors
    Proceedings of the National Academy of Sciences
    • Vol. 74
    • No. 12

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