In vivo regeneration of red cell 2, 3-diphosphoglycerate following transfusion of DPG-depleted AS-1, AS-3 and CPDA-1 red cells
Summary
Regeneration of 2, 3-diphosphoglycerate (DPG) was determined following transfusion of DPG-depleted group O red cells into group A recipients. Blood from five donors was stored in the adenine-containing solutions CPDA-1, AS-1 or AS-3 for 35 d at 4°C. Post-transfusion red cell DPG and ATP were measured in separated group O red cells over a 7 d period. The studies confirmed rapid in vivo DPG regeneration with ≥ 50% of the maximum level being achieved within 7 h. An average of 95% of the recipients’ pre-transfusion DPG level was achieved by 72 h and by 7 d mean (±SEM) DPG levels relative to recipient's pre-transfusion DPG averaged 84% (± 13%), 92% (± 17%) and 84% (± 21%) for CPDA-1, AS-1 and AS-3 red cells, respectively. Results were comparable to those previously reported for blood stored in ACD for 15-20 d (Valeri & Hirsch, 1969; Beutler & Wood, 1969). The immediate regeneration rate, V, closely approximated first order regeneration kinetics with AS-3 red cells exhibiting double the rate of CPDA-1 red cells (P<0·001). AS-1 red cells exhibited an intermediate rate of regeneration which was not significantly different compared to either CPDA-1 or AS-3 (P>0·05). V exhibited a significant (P<0·05) positive correlation with ATP levels 5-7 h post-infusion. ATP regeneration of the infused cells was rapid with a mean increase of 1·2 μmol/g Hb above post-storage levels being achieved 1 h following transfusion.