Volume 71, Issue 1 p. 131-136

In vivo regeneration of red cell 2, 3-diphosphoglycerate following transfusion of DPG-depleted AS-1, AS-3 and CPDA-1 red cells

Andrew Heaton

Andrew Heaton

Tidewater Red Cross Blood Services and Department of Pathology, Eastern Virginia Medical School, Norfolk, Virginia

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Thais Keegan

Thais Keegan

Tidewater Red Cross Blood Services and Department of Pathology, Eastern Virginia Medical School, Norfolk, Virginia

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Stein Holme

Corresponding Author

Stein Holme

Tidewater Red Cross Blood Services and Department of Pathology, Eastern Virginia Medical School, Norfolk, Virginia

Dr Stein Holme, Scientific Director, Research, American Red Cross Blood Services, Tidewater Region, 700 Olney Road, Norfolk, VA 23507, U.S.A.Search for more papers by this author
First published: January 1989
Citations: 137

Summary

Regeneration of 2, 3-diphosphoglycerate (DPG) was determined following transfusion of DPG-depleted group O red cells into group A recipients. Blood from five donors was stored in the adenine-containing solutions CPDA-1, AS-1 or AS-3 for 35 d at 4°C. Post-transfusion red cell DPG and ATP were measured in separated group O red cells over a 7 d period. The studies confirmed rapid in vivo DPG regeneration with ≥ 50% of the maximum level being achieved within 7 h. An average of 95% of the recipients’ pre-transfusion DPG level was achieved by 72 h and by 7 d mean (±SEM) DPG levels relative to recipient's pre-transfusion DPG averaged 84% (± 13%), 92% (± 17%) and 84% (± 21%) for CPDA-1, AS-1 and AS-3 red cells, respectively. Results were comparable to those previously reported for blood stored in ACD for 15-20 d (Valeri & Hirsch, 1969; Beutler & Wood, 1969). The immediate regeneration rate, V, closely approximated first order regeneration kinetics with AS-3 red cells exhibiting double the rate of CPDA-1 red cells (P<0·001). AS-1 red cells exhibited an intermediate rate of regeneration which was not significantly different compared to either CPDA-1 or AS-3 (P>0·05). V exhibited a significant (P<0·05) positive correlation with ATP levels 5-7 h post-infusion. ATP regeneration of the infused cells was rapid with a mean increase of 1·2 μmol/g Hb above post-storage levels being achieved 1 h following transfusion.

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