Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
Abstract
In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratorysyndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of SARS-CoV is 29,727 nucleotides in length and has 11 open reading frames, and its genome organization is similar to that of other coronaviruses. Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closelyrelated to anyof the previouslycharacterized coronaviruses.
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References and Notes
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Materials and methods are available as supporting material on Science Online.
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26
Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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As of this writing, complete genomic sequences of three additional SARS-CoV isolates were available at GenBank (Tor-2 strain, Canada, accession no. ay274119; CUHK-W1 isolate, Hong Kong, accession no. ay278554; and HKU-39849 isolate, Hong Kong, accession no. ay278491). A comparison of these sequences to the sequence described in this paper is shown in table S3.
30
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The authors thank the WHO SARS Aetiology Laboratory Investigation Group (Bernhard-Nocht Institute, Hamburg, Germany; Erasmus Universiteit, National Influenza Centre, Rotterdam, Netherlands; Federal Microbiology Laboratories for Health Canada, Winnipeg, Canada; Institut für Virologie, Marburg Germany; Frankfurt A. M. University Hospital, Klinikum der Johann Wolfgang Goethe-Universität, Frankfurt, Germany; Chinese Center for Disease Control, Beijing, China; Public Health Laboratory Service Central Public Health Laboratory, London; Prince of Wales Hospital, Hong Kong; National Institute of Infectious Disease, Tokyo, Japan; The Chinese University of Hong Kong, Hong Kong; Government Virus Unit, Hong Kong; Queen Mary Hospital, Hong Kong; and Institute Pasteur, Paris, France) for the open collaboration and sharing of information; Centers for Disease Control (CDC) Laboratory Partners Group for support and suggestions; the Coronavirology Partners Group (S. C. Baker, R. Baric, D. A. Brian, D. Cavanagh, M. R. Denison, M. S. Diamond, B. G. Hogue, K. V. Holmes, J. Leibowitz, S. Perlman, L. J. Saif, L. Sturman, and S. R. Weiss) for many helpful reagents, guidance and discussion; B. W. J. Mahy for advice and discussions and for organizing the Laboratory Partners Conferences; S. Emery for technical support; J. Osborne and S. Sammons for help with the figures; and C. Chesley for editorial assistance. M-h.C. is supported by a CDC/Georgia State University interagency agreement.
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Science
Volume 300 | Issue 5624
30 May 2003
30 May 2003
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American Association for the Advancement of Science.
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Submission history
Received: 18 April 2003
Accepted: 30 April 2003
Published in print: 30 May 2003
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www.sciencemag.org/cgi/content/full/1085952/DC1
Materials and Methods
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Tables S1 to S3
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