Advertisement

Abstract

The genome of the yeast Saccharomyces cerevisiae has been completely sequenced through a worldwide collaboration. The sequence of 12,068 kilobases defines 5885 potential protein-encoding genes, approximately 140 genes specifying ribosomal RNA, 40 genes for small nuclear RNA molecules, and 275 transfer RNA genes. In addition, the complete sequence provides information about the higher order organization of yeast's 16 chromosomes and allows some insight into their evolutionary history. The genome shows a considerable amount of apparent genetic redundancy, and one of the major problems to be tackled during the next stage of the yeast genome project is to elucidate the biological functions of all of these genes.

Get full access to this article

View all available purchase options and get full access to this article.

REFERENCES AND NOTES

1
Oliver S. G., et al., Nature 357381992.
2
Dujon B., et al., ibid. 3693711994.
3
Johnston M., et al., Science 26520771994.
4
Feldmann H., et al., EMBO J. 1357951994.
5
Bussey H., et al., Proc. Natl. Acad. Sci. U.S.A. 9238091995.
6
Murakami Y., et al., Nature Genet. 102611995.
7
Galibert F., et al., EMBO J. 1520311996.
8
Barrell B., et al. in preparation.
9
Barrell B., et al. in preparation.
10
Bussey H., et al. in preparation.
11
Dietrich F., et al. in preparation.
12
Dujon B., et al. in preparation.
13
Jacq C., et al. in preparation.
14
Johnston M., et al. in preparation.
15
Philippsen P., et al. in preparation.
16
Tettelin H., et al. in preparation.
17
Rothstein R. J., Methods Enzymol. 1012021983.
18
Baudin A., Ozier-Kalogeropoulos O., Denouel A., Lacroute F., Cullin C., Nucleic Acids Res. 2133291993.
19
Wach A., Brachat A., Pohlmann R., Philippsen P., Yeast 1017931994.
20
Mortimer R. K., Contopoulou C. R., King J. S., ibid. 88171992.
21
Dujon B., Trends Genet. 122631996.
22
Hodgkin J. R. H. A. Plasterk, R. H. WaterstonScience 2704101994.
24
Planta R. J., Goncalves P. M., Mager W. H., Biochem. Cell Biol. 738251996.
25
Rymond B. C., Rosbash M., The Molecular Biology of the Yeast Saccharomyces: Gene Expression, Jones E. W., Pringle J. R., Broach J. R.Cold Spring Harbor Laboratory PressCold Spring Harbor, NY1992143-192.
26
Fink G. R., Cell 4951987.
27
Sharp P. M., Lloyd A. T., Nucleic Acids Res. 211791993.
28
Zenvirth D., et al., EMBO J. 1134411992.
29
De B. Massy, V. Rocco, A. Nicolasibid. 1445891995.
30
Kaback D. B., Steensma H. Y. P. de JongeProc. Natl. Acad. Sci. U.S.A. 8636941989.
31
Hieter P., Mann C., Snyder M., Davis R. W., Cell 403811985.
32
Murray A. W., Schultes N. P., Szostak J. W, ibid. 455291986.
33
Steensma H. Y., Crowley J. C., Kaback D. B., Mol. Cell. Biol. 728941987.
34
Charron M. J., Read E., Haut S. R., Michels C. A., Genetics 1223071989.
35
Carlson M., Celenza J. L., Eng F. J., Mol. Cell. Biol. 54101985.
36
Naumov G. I., Naumova E. S., Louis E. J., Yeast 114811995.
37
Chan C., Tye B.-K., Proc. Natl. Acad. Sci. U.S.A. 7763291980.
38
Link A. J., Olson M. V., Genetics 1276811991.
39
Voytas D. F., Boeke J. D., Nature 3587171992.
40
Danilevskaya O., Lofsky A., Pardue M.-L, Mol. Cell. Biol. 35S831992.
41
Biessmann H., et al., ibid. 1239101992.
42
Foury F.personal communication.
43
Louis E. J., Yeast 1115531995.
44
Louis E. J., Haber J. E., Genetics 1315591992.
45
Grieder C. W, The Eukaryotic Genome: Organisation and Regulation, Broda P. M. A., Oliver S. G., Sims P. F. G.Cambridge Univ. PressCambridge199331-42.
46
Wicksteed B. L., et al., Yeast 10391992.
47
Eigel A., Feldmann H., EMBO J. 112451982.
48
Gafner J., De Robertis E. M., Philippsen P., ibid. 25831983.
49
Warmington J. R., et al., Nucleic Acids Res. 1434751986.
50
Warmington J. R., Green R. P., Newlon C. S., Oliver S. G., ibid. 1589631987.
51
Hauber J., Stucka R., Krieg R., Feldmann H., ibid. 16106231988.
52
Lochmüller H., Stucka R., Feldmann H., Curr. Genet. 162471989
53
Ji H., et al., Cell 7310071993.
54
Wilkins M. R., et al., Bio/Technology 14611996.
55
Oliver S. G., Nature 3795971996.
56
Mewes H. W. personal communication.
57
Svetlov V. V., Cooper T. G., Yeast 1114391995.
58
Nelissen B., Mordant P., Jonniaux J. L., De Wachter R., Goffeau A., FEBS Lett. 377321995.
59
Sagliocco S., et al., Yeastin press.
60
Ushinsky S. C., et al., ibid.in press.
62
Bassett D. E., Boguski M. S., Hieter P., Nature 3795891996; http://www.ncbi.nlm.nih.gov/XREFdb/.
63
Ohno S., Evolution Through Gene DuplicationAllen and UnwinLondon1970.
64
Olson M. V., The Molecular Biology of the Yeast Saccharomyces: Genome Dynamics Protein Synthesis, and Energetics, Broach J. R., Pringle J. R., Jones E. W.Cold Spring Harbor Laboratory PressCold Spring Harbor, NY19911-39.
65
Viswanathan M., et al., Gene 1481491994.
66
Purnelle B., Goffeau A., Yeastin press.
67
Melnick L., Sherman F., J. Mol. Biol. 2333721993.
68
Lalo D., Stettler S., Mariotte S., Slonimski P. P., Thuriaux P., C. R. Acad. Sci. Paris 3163671993.
69
Bécam A.-M., Jia Y., Slonimski P. P., Herbert C. J., Yeast 11S3001995
70
Mortimer R. K., Johnston J. R., Genetics 113351989.
71
Mortimer R. K., Torok T., Romano P., Suzzi G., Polsinelli M., Yeast 11S5741995.
72
Oliver S. G., Trends Genet. 122411996.
73
Langle-Rouault F., Jacobs E., Nucleic Acids Res. 2330791995.
74
Guldener U., Heck S., Fiedler T., Beinhauer J., Hegemann J. H., ibid. 2425191996.
75
Fleischmann R. D., et al., Science 2694961995.
76
Frazer C. M., et al., ibid. 2703971995.
77
Bult C., et al., ibid. 27310581996.
78
Riles L., et al., Genetics 134811993.
79
Thierry A., Gaillon L., Galibert F., Dujon B., Yeast 111211995.
80
Hoheisel J. D., et al., Cell 731091993.
81
Mizukami T., et al., ibid.: 121.
82
Chu W. S., Magee B. B., Magee P. T., J. Bacteriol. 17566371993.
84
Altman-Jöhl R., Philippsen P., Molec. Gen. Genet. 250691996
85
Russell P., Nurse P., Cell 457811986.
86
Walsh S., Barrell B., Trends Genet. 122761996.

(0)eLetters

eLetters is a forum for ongoing peer review. eLetters are not edited, proofread, or indexed, but they are screened. eLetters should provide substantive and scholarly commentary on the article. Embedded figures cannot be submitted, and we discourage the use of figures within eLetters in general. If a figure is essential, please include a link to the figure within the text of the eLetter. Please read our Terms of Service before submitting an eLetter.

Log In to Submit a Response

No eLetters have been published for this article yet.

Information & Authors

Information

Published In

Science
Volume 274 | Issue 5287
25 October 1996

Submission history

Published in print: 25 October 1996

Permissions

Request permissions for this article.

Authors

Affiliations

A. Goffeau*
A. Goffeau and H. Tettelin, Université Catholique de Louvain, Unité de Biochimie Physiologique, Place Croix du Sud, 2/20, 1348 Louvain-la-Neuve, Belgium.
B. G. Barrell
B. G. Barrell, Sanger Centre, Hinxton Hall, Hinxton, Cambridge CB10 1SA, UK.
H. Bussey
H. Bussey, Department of Biology, McGill University, 1205 Docteur Penfield Avenue, Montreal, H3A 1B1, Canada.
R. W. Davis
R. W. Davis, Department of Biochemistry, Stanford University, Beckman Center, Room B400, Stanford, CA 94305-5307, USA.
B. Dujon
B. Dujon, Unité de Génétique Moléculaire des Levures (URA1149 CNRS and UPR927 Université Pierre et Marie Curie), Institut Pasteur, 25 Rue du Docteur Roux, 75724 Paris-Cedex 15, France.
H. Feldmann
H. Feldmann, Universität München, Institut für Physiologische Chemie, Schillerstrasse, 44, 80336 München, Germany.
F. Galibert
F. Galibert, Laboratoire de Biochimie Moleculaire, UPR 41-Faculté de Médecine, CNRS, 2 Avenue du Professeur Léon Bernard, 35043 Rennes Cedex, France.
J. D. Hoheisel
J. D. Hoheisel, Molecular Genetic Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld, 506, 69120 Heidelberg, Germany.
C. Jacq
C. Jacq, Génétique Moleculaire, Ecole Normale Supérieure, CNRS URA 1302, 46 Rue d'Ulm, 75230 Paris Cedex 05, France.
M. Johnston
M. Johnston, Department of Genetics, Box 8232, Washington University Medical School, 4566 Scott Avenue, St. Louis, MO 63110, USA.
E. J. Louis
E. J. Louis, Yeast Genetics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, UK.
H. W. Mewes
H. W. Mewes, Max-Planck-Institut für Biochemie, Martinsried Institute for Protein Sciences (MIPS), Am Klopferspitz 18A, 82152 Martinsried, Germany.
Y. Murakami
Y. Murakami, Tsukuba Life Science Center, Division of Human Genome Research, RIKEN, Koyaday Tsukuba Science City 3-1-1, 305 Ibaraki, Japan.
P. Philippsen
P. Philippsen, Institute for Applied Microbiology, Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland.
H. Tettelin
A. Goffeau and H. Tettelin, Université Catholique de Louvain, Unité de Biochimie Physiologique, Place Croix du Sud, 2/20, 1348 Louvain-la-Neuve, Belgium.
S. G. Oliver
S. G. Oliver, Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), Post Office Box 88, Manchester M60 1QD, UK.

Notes

*
To whom correspondence should be addressed.

Metrics & Citations

Metrics

Article Usage

Altmetrics

Citations

Cite as

Export citation

Select the format you want to export the citation of this publication.

Cited by

  1. An orthologous gene coevolution network provides insight into eukaryotic cellular and genomic structure and function, Science Advances, 8, 18, (2022)./doi/10.1126/sciadv.abn0105
    Abstract
  2. Genomics and evolution of protein phosphatases, Science Signaling, 10, 474, (2021)./doi/10.1126/scisignal.aag1796
    Abstract
  3. “Perfect” designer chromosome V and behavior of a ring derivative, Science, 355, 6329, (2021)./doi/10.1126/science.aaf4704
    Abstract
  4. Design of a synthetic yeast genome, Science, 355, 6329, (1040-1044), (2021)./doi/10.1126/science.aaf4557
    Abstract
  5. Making Sense of the Sequence, Science, 291, 5507, (1257-1260), (2021)./doi/10.1126/science.291.5507.1257
    Abstract
  6. Comparative Genomics of the Eukaryotes, Science, 287, 5461, (2204-2215), (2021)./doi/10.1126/science.287.5461.2204
    Abstract
  7. Genome Sequence of the Nematode C. elegans: A Platform for Investigating Biology, Science, 282, 5396, (2012-2018), (2021)./doi/10.1126/science.282.5396.2012
    Abstract
  8. Functional Genomics: It's All How You Read It, Science, 278, 5338, (601-602), (2021)./doi/10.1126/science.278.5338.601
    Abstract
  9. Total Synthesis of a Functional Designer Eukaryotic Chromosome, Science, 344, 6179, (55-58), (2021)./doi/10.1126/science.1249252
    Abstract
  10. Very Low Gene Duplication Rate in the Yeast Genome, Science, 306, 5700, (1367-1370), (2021)./doi/10.1126/science.1102033
    Abstract
  11. See more
Loading...

View Options

Check Access

Log in to view the full text

AAAS ID LOGIN

AAAS login provides access to Science for AAAS Members, and access to other journals in the Science family to users who have purchased individual subscriptions.

Log in via OpenAthens.
Log in via Shibboleth.

More options

Purchase digital access to this article

Download and print this article for your personal scholarly, research, and educational use.

Purchase this issue in print

Buy a single issue of Science for just $15 USD.

View options

PDF format

Download this article as a PDF file

Download PDF

Full Text

FULL TEXT

Media

Figures

Multimedia

Tables

Share

Share

Share article link

Share on social media