Detection and Quantification of Gene Expression in Environmental Bacteriology
QUANTIFICATION OF TOTAL RNA CONCENTRATIONS
QUANTIFICATION OF SPECIFIC MESSENGER RNAs
RT-PCR: the basics.
cRT-PCR.
(i) Internal standards.
(ii) General considerations.
(iii) Environmental applications of cRT-PCR.
Real-time PCR technology and environmental analysis.
(i) Real-time PCR instrumentation.
(ii) Detection chemistries. (a) Hydrolysis and TaqMan probes.
(b) Scorpions.
(c) DNA binding dyes.
(d) Molecular beacons.
(e) Hybridization probes.
(f) LUX fluorogenic primers.
(iii) Real-time PCR data analysis.
(iv) Microarray analysis: the technology of the future?
(v) Applications in environmental monitoring.
WHAT DOES THE FUTURE HOLD?
Manufacturer | Real-time system | Normal sample format | Maximum no. of samples | Dynamic range (orders of magnitude) |
---|---|---|---|---|
Applied Biosystems | ABI Prism 7000 | Microplate | 96 | 5 |
ABI Prism 7900 HT | Microplate | 384 | 5 | |
Roche Biochemicals | LightCycler | Capillaries | 32 | 7 |
Corbett Research | Rotor-Gene 3000 | Strip tubes | 72 | 7 |
Bio-Rad | iCycler | Microplate, strip tubes | 96 | 6 |
MyiQ | Microplate, strip tubes | 96 | 8 | |
Cepheid | SmartCycler | Microplate, strip tubes | 96 | 8 |
Stratagene | Mx3000P | Microplate, strip tubes | 96 | 7 |
Mx4000P | Microplate, strip tubes | 96 | 7 | |
MJ Research | Opticon | Microplate, strip tubes | 96 | 8 |
Opticon 2 | Microplate, strip tubes | 96 | 10 | |
Techne | Quantica | Microplate | 96 | 6 |
REFERENCES
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