ACE2 Receptor Expression and Severe Acute Respiratory Syndrome Coronavirus Infection Depend on Differentiation of Human Airway Epithelia
ABSTRACT
MATERIALS AND METHODS
Epithelial cell culture.
Immunoblot analysis.
Immunofluorescence staining.
Surface biotinylation.
Scanning electron microscopy (SEM).
Real-time reverse transcription-PCR mRNA analysis.
Adenoviral vectors.
Preparation of SARS S protein-pseudotyped lentivirus and infection of airway epithelia.
β-Galactosidase activity assays.
Preparation of wild-type SARS-CoV and infection of airway epithelia.
RESULTS
ACE2 expression in human airway epithelia.
SARS-CoV infection of human airway epithelia.
DISCUSSION
Virion egress type | Egress value (PFU/ml) for specimen no.: | ||||
---|---|---|---|---|---|
1 | 2 | 3 | |||
Apical | 2.5 × 104 | 5 × 104 | 3 × 104 | ||
Basolateral | 15 | 20 | 5 |
Acknowledgments
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