Annals of the New York Academy of Sciences

Volume 1067, Issue 1 p. 500-505

Severe Acute Respiratory Syndrome (SARS)

Development of Diagnostics and Antivirals

MORTEN DRÆBY SØRENSEN,

MORTEN DRÆBY SØRENSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

BRIAN SØRENSEN,

BRIAN SØRENSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

REGINA GONZALEZ-DOSAL,

REGINA GONZALEZ-DOSAL

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

CONNIE JENNING MELCHJORSEN,

CONNIE JENNING MELCHJORSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

JENS WEIBEL,

JENS WEIBEL

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

JING WANG,

JING WANG

Beijing Genomics Institute, Beijing, China

Search for more papers by this author

CHEN WIE JUN,

CHEN WIE JUN

Beijing Genomics Institute, Beijing, China

Search for more papers by this author

YANG HUANMING,

YANG HUANMING

Beijing Genomics Institute, Beijing, China

Search for more papers by this author

PETER KRISTENSEN,

PETER KRISTENSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

MORTEN DRÆBY SØRENSEN,

MORTEN DRÆBY SØRENSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

BRIAN SØRENSEN,

BRIAN SØRENSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

REGINA GONZALEZ-DOSAL,

REGINA GONZALEZ-DOSAL

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

CONNIE JENNING MELCHJORSEN,

CONNIE JENNING MELCHJORSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

JENS WEIBEL,

JENS WEIBEL

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

JING WANG,

JING WANG

Beijing Genomics Institute, Beijing, China

Search for more papers by this author

CHEN WIE JUN,

CHEN WIE JUN

Beijing Genomics Institute, Beijing, China

Search for more papers by this author

YANG HUANMING,

YANG HUANMING

Beijing Genomics Institute, Beijing, China

Search for more papers by this author

PETER KRISTENSEN,

PETER KRISTENSEN

Department of Molecular Biology, University of Aarhus, Aarhus, Denmark

Search for more papers by this author

First published: 10 May 2006

https://doi.org/10.1196/annals.1354.072

Citations: 34

Address for correspondence: Dr. Peter Kristensen, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK8000 Aarhus−C, Denmark. Voice: +45 8942 5032; fax: +45 8612 3178.
e-mail: [email protected]

Read the full text

About

PDF

Tools

Share a link

Email
Facebook
Twitter
LinkedIn
Reddit
Wechat

Abstract

Abstract: The previously unknown coronavirus that caused severe acute respiratory syndrome (SARS-CoV) affected more than 8,000 persons worldwide and was responsible for more than 700 deaths during the first outbreak in 2002–2003. For reasons unknown, the SARS virus is less severe and the clinical progression a great deal milder in children younger than 12 years of age. In contrast, the mortality rate can exceed 50% for persons at or above the age of 60. As part of the Sino-European Project on SARS Diagnostics and Antivirals (SEPSDA), an immune phage-display library is being created from convalescent patients in a phagemid system for the selection of single-chain fragment variables (scFv) antibodies recognizing the SARS-CoV.

REFERENCES

1 World Health Organization . 2003. Severe acute respiratory syndrome (SARS): multi-country outbreak. http://www.who.int/csr/don/2003_03-16/en/.

Google Scholar
2 Rosling, L. & M. Rosling. 2003. Pneumonia causes panic in Guangdong province. Br. Med. J. 326: 416.
10.1136/bmj.326.7386.416
PubMedWeb of Science®Google Scholar
3 Drosten, C. et al . 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348: 1967–1976.
10.1056/NEJMoa030747
CASPubMedWeb of Science®Google Scholar
4 Ruan, Y.J. et al . 2003. Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet 361: 1779–1785.
10.1016/S0140-6736(03)13414-9
CASPubMedWeb of Science®Google Scholar
5 Peiris, J.S. et al . 2003. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 361: 1319–1325.
10.1016/S0140-6736(03)13077-2
CASPubMedWeb of Science®Google Scholar
6 Rota, P.A. et al . 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300: 1394–1399.
10.1126/science.1085952
CASPubMedWeb of Science®Google Scholar
7 Marra, M.A. et al . 2003. The genome sequence of the SARS-associated coronavirus. Science 300: 1399–1404.
10.1126/science.1085953
CASPubMedWeb of Science®Google Scholar
8 Ksiazek, T.G. et al . 2003. A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med. 348: 1953–1966.
10.1056/NEJMoa030781
CASPubMedWeb of Science®Google Scholar
9 Fouchier, R.A. et al . 2003. Aetiology: Koch's postulates fulfilled for SARS virus. Nature 423: 240.
10.1038/423240a
CASPubMedWeb of Science®Google Scholar
10 Thiel, V. et al . 2003. Mechanisms and enzymes involved in SARS coronavirus genome expression. J. Gen. Virol. 84: 2305–2315.
10.1099/vir.0.19424-0
CASPubMedWeb of Science®Google Scholar
11 Leung, C.W. et al . 2004. Severe acute respiratory syndrome among children. Pediatrics 113: e535–e543.
10.1542/peds.113.6.e535
PubMedWeb of Science®Google Scholar
12 Liang, W. et al . 2004. Severe acute respiratory syndrome, Beijing, 2003. Emerg. Infect. Dis. 10: 25–31.
10.3201/eid1001.030553
PubMedWeb of Science®Google Scholar
13 Donnelly, C.A. et al . 2003. Epidemiological determinants of spread of causal agent of severe acute respiratory syndrome in Hong Kong. Lancet 361: 1761–1766.
10.1016/S0140-6736(03)13410-1
PubMedWeb of Science®Google Scholar
14 Tan, Y.J., S.G. Lim & W. Hong. 2005. Characterization of viral proteins encoded by the SARS-coronavirus genome. Antiviral Res. 65: 69–78.
10.1016/j.antiviral.2004.10.001
CASPubMedWeb of Science®Google Scholar
15 Krokhin, O. et al . 2003. Mass spectrometric characterization of proteins from the SARS virus: a preliminary report. Mol. Cell Proteomics 2: 346–356.
10.1074/mcp.M300048-MCP200
CASPubMedWeb of Science®Google Scholar
16 Li, G., X. Chen & A. Xu. 2003. Profile of specific antibodies to the SARS-associated coronavirus. N. Engl. J. Med. 349: 508–509.
10.1056/NEJM200307313490520
PubMedWeb of Science®Google Scholar
17 Chen, W. et al . 2004. Antibody response and viraemia during the course of severe acute respiratory syndrome (SARS)-associated coronavirus infection. J. Med. Microbiol. 53: 435–438.
10.1099/jmm.0.45561-0
CASPubMedWeb of Science®Google Scholar
18 Wong, S.K. et al . 2004. A 193-amino acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 2. J. Biol. Chem. 279: 3197–3201.
10.1074/jbc.C300520200
CASPubMedWeb of Science®Google Scholar
19 Sui, J. et al . 2004. Potent neutralization of severe acute respiratory syndrome (SARS) coronavirus by a human mAb to S1 protein that blocks receptor association. Proc. Natl. Acad. Sci. USA 101: 2536–2541.
10.1073/pnas.0307140101
CASPubMedWeb of Science®Google Scholar
20 Yi, C.E. et al . 2005. Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. J. Virol. 79: 11638–11646.
10.1128/JVI.79.18.11638-11646.2005
CASPubMedWeb of Science®Google Scholar
21 Anand, K. et al . 2003. Coronavirus main proteinase (3CLpro) structure: basis for design of anti-SARS drugs. Science 300: 1763–1767.
10.1126/science.1085658
CASPubMedWeb of Science®Google Scholar
22 Zhai, Y. et al . 2005. Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat. Struct. Mol. Biol. 12: 980–986.
10.1038/nsmb999
CASPubMedWeb of Science®Google Scholar
23 Yang, H. et al . 2003. The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. Proc. Natl. Acad. Sci. USA 100: 13190–13195.
10.1073/pnas.1835675100
CASPubMedWeb of Science®Google Scholar
24 Chen, L. et al . 2005. Cinanserin is an inhibitor of the 3C-like proteinase of severe acute respiratory syndrome coronavirus and strongly reduces virus replication in vitro. J. Virol. 79: 7095–7103.
10.1128/JVI.79.11.7095-7103.2005
CASPubMedWeb of Science®Google Scholar
25 Wen, K. et al . 2004. Preparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein. Di Yi Jun Yi Da Xue Xue Bao. 24: 1–6.
10.3736/jcim20040101
PubMedGoogle Scholar
26 Lin, Y. et al . 2003. Identification of an epitope of SARS-coronavirus nucleocapsid protein. Cell Res. 13: 141–145.
10.1038/sj.cr.7290158
CASPubMedWeb of Science®Google Scholar
27 Liu, H. et al . 2004. Recombinant scFv antibodies against E protein and N protein of severe acute respiratory syndrome virus. Acta. Biochim. Biophys. Sin. (Shanghai). 36: 541–547.
10.1093/abbs/36.8.541
PubMedWeb of Science®Google Scholar
28 Zhong, X. et al . 2005. B-cell responses in patients who have recovered from severe acute respiratory syndrome target a dominant site in the S2 domain of the surface spike glycoprotein. J. Virol. 79: 3401–3408.
10.1128/JVI.79.6.3401-3408.2005
CASPubMedWeb of Science®Google Scholar
29 Van Den Brink, E.N. et al . 2005. Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus. J. Virol. 79: 1635–1644.
10.1128/JVI.79.3.1635-1644.2005
CASPubMedWeb of Science®Google Scholar
30 Duan, J. et al . 2005. A human SARS-CoV neutralizing antibody against epitope on S2 protein. Biochem. Biophys. Res. Commun. 333: 186–193.
10.1016/j.bbrc.2005.05.089
CASPubMedWeb of Science®Google Scholar
31 Smith, G.P. 1985. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228: 1315–1317.
10.1126/science.4001944
CASPubMedWeb of Science®Google Scholar
32 McCafferty, J. et al . 1990. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552–554.
10.1038/348552a0
CASPubMedWeb of Science®Google Scholar
33 Barbas, C.F. III et al . 1991. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. USA 88: 7978–7982.
10.1073/pnas.88.18.7978
CASPubMedWeb of Science®Google Scholar
34 Hoogenboom, H.R. et al . 1991. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19: 4133–4137.
10.1093/nar/19.15.4133
CASPubMedWeb of Science®Google Scholar
35 Kang, A.S. et al . 1991. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proc. Natl. Acad. Sci. USA 88: 4363–4366.
10.1073/pnas.88.10.4363
CASPubMedWeb of Science®Google Scholar
36 Marks, J.D. et al . 1991. By-passing immunization: human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222: 581–597.
10.1016/0022-2836(91)90498-U
CASPubMedWeb of Science®Google Scholar
37 Clackson, T. et al . 1991. Making antibody fragments using phage display libraries. Nature 352: 624–628.
10.1038/352624a0
CASPubMedWeb of Science®Google Scholar
38 Hust, M. & S. Dubel. 2004. Mating antibody phage display with proteomics. Trends Biotechnol. 22: 8–14.
10.1016/j.tibtech.2003.10.011
CASPubMedWeb of Science®Google Scholar
39 Kramer, R.A. et al . 2005. The human antibody repertoire specific for rabies virus glycoprotein as selected from immune libraries. Eur. J. Immunol. 35: 2131–2145.
10.1002/eji.200526134
CASPubMedWeb of Science®Google Scholar
40 Kausmally, L. et al . 2004. Neutralizing human antibodies to varicella-zoster virus (VZV) derived from a VZV patient recombinant antibody library. J. Gen. Virol. 85: 3493–3500.
10.1099/vir.0.80406-0
CASPubMedWeb of Science®Google Scholar
41 Kim, S.J. et al . 2004. Neutralizing human monoclonal antibodies to hepatitis A virus recovered by phage display. Virology 318: 598–607.
10.1016/j.virol.2003.10.014
CASPubMedWeb of Science®Google Scholar
42 Schofield, D.J. et al . 2003. Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine. Vaccine 22: 257–267.
10.1016/j.vaccine.2003.07.008
CASPubMedWeb of Science®Google Scholar
43 De Carvalho Nicacio, C. et al . 2002. Neutralizing human Fab fragments against measles virus recovered by phage display. J. Virol. 76: 251–258.
10.1128/JVI.76.1.251-258.2002
PubMedWeb of Science®Google Scholar
44 Nguyen, H. et al . 2000. Efficient generation of respiratory syncytial virus (RSV)-neutralizing human MoAbs via human peripheral blood lymphocyte (hu-PBL)-SCID mice and scFv phage display libraries. Clin. Exp. Immunol. 122: 85–93.
10.1046/j.1365-2249.2000.01345.x
CASPubMedWeb of Science®Google Scholar

Citing Literature

Volume1067, Issue1

Understanding and Modulating Aging

May 2006

Pages 500-505

Severe Acute Respiratory Syndrome (SARS)

Development of Diagnostics and Antivirals

Abstract

REFERENCES

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Severe Acute Respiratory Syndrome (SARS)

Development of Diagnostics and Antivirals

Abstract

REFERENCES

Citing Literature

References

Related

Information