Phytolaccasaponin B, E and G, the major saponins of Phytolacca americana L. (Phytolaccaceae), have been isolated. The structures of phytolaccasaponin B, E and G have been established to be phytolaccagenin 3-O-[β-D-glucopyranosyl (1→4)-β-D-xylopyranoside]-28-O-β-D-glucopyranoside (4), phytolaccagenin 3-O-β-D-glucopyranosyl (1→4)-β-D-xylopyranoside (5) and phytolaccagenin 3-O-β-D-xylopyranoside (6), respectively. Phytolaccatoxin reported by Stout, et al. was assumed to be a mixture of more than three saponins, but the main saponin was identified as 5 by thin-layer chromatography.