Expasy logo

ENZYME

ENZYME entry: EC 1.14.13.39

Accepted Name
nitric-oxide synthase (NADPH)
Alternative Name(s)
endothelium-derived relaxation factor-forming enzyme
endothelium-derived relaxing factor synthase
NADPH-diaphorase
nitric-oxide synthetase
NO synthase
Reaction catalysed
H(+) + 2 L-arginine + 3 NADPH + 4 O2 <=> 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide
Comment(s)
  • The enzyme consists of linked oxygenase and reductase domains.
  • The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain.
  • Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center.
  • The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. cf. EC 1.14.14.47.
Cross-references
BRENDA 1.14.13.39
EC2PDB 1.14.13.39
ExplorEnz 1.14.13.39
PRIAM enzyme-specific profiles 1.14.13.39
KEGG Ligand Database for Enzyme Nomenclature 1.14.13.39
IUBMB Enzyme Nomenclature 1.14.13.39
IntEnz 1.14.13.39
MEDLINE Find literature relating to 1.14.13.39
MetaCyc 1.14.13.39
Rhea expert-curated reactions 1.14.13.39
UniProtKB/Swiss-Prot
Q66GP9, NOA1_ARATH P29475, NOS1_HUMAN Q9Z0J4, NOS1_MOUSE
O19132, NOS1_RABIT P29476, NOS1_RAT Q29498, NOS1_SHEEP
Q27995, NOS2_BOVIN O62699, NOS2_CANLF Q28314, NOS2_CAPHI
Q92037, NOS2_CARAU O54705, NOS2_CAVPO Q90703, NOS2_CHICK
P35228, NOS2_HUMAN O46660, NOS2_MACMU P29477, NOS2_MOUSE
Q92091, NOS2_ONCMY P79290, NOS2_PIG O19114, NOS2_RABIT
Q06518, NOS2_RAT P29473, NOS3_BOVIN Q9TUX8, NOS3_CANLF
P97270, NOS3_CAVPO P29474, NOS3_HUMAN P70313, NOS3_MOUSE
Q28969, NOS3_PIG Q62600, NOS3_RAT P79209, NOS3_SHEEP
B1B557, NOSL_BOMMO O61608, NOS_ANOST Q8T8C0, NOS_BOMMO
Q27571, NOS_DROME O61309, NOS_LYMST Q6YPG5, NOS_ORYSJ
Q26240, NOS_RHOPR Q9I9M2, NOS_SQUAC

View entry in original ENZYME format
View entry in raw text format (no links)
All UniProtKB/Swiss-Prot entries referenced in this entry, with possibility to download in different formats, align etc.
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.13.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.14.-.-
All ENZYME / UniProtKB/Swiss-Prot entries corresponding to 1.-.-.-