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ENZYME

ENZYME entry: EC 1.4.1.13

Accepted Name
glutamate synthase (NADPH)
Alternative Name(s)
glutamate (reduced nicotinamide adenine dinucleotide phosphate) synthase
glutamate synthetase (NADP)
glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP)
glutamine-ketoglutaric aminotransferase
GOGAT
L-glutamate synthase
L-glutamate synthetase
NADPH-dependent glutamate synthase
NADPH-glutamate synthase
Reaction catalysed
2 L-glutamate + NADP(+) <=> 2-oxoglutarate + H(+) + L-glutamine + NADPH
Comment(s)
  • The reaction takes place in the opposite direction.
  • The protein is composed of two subunits, alpha and beta.
  • The alpha subunit is composed of two domains, one hydrolyzing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.4).
  • The beta subunit transfers electrons from the cosubstrate.
  • The NH3 is channeled through a 31 A channel in the active protein.
  • In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced.
  • In the intact alphabeta complex, ammonia production only takes place as part of the overall reaction.
  • Formerly EC 2.6.1.53.
Cross-references
BRENDA 1.4.1.13
EC2PDB 1.4.1.13
ExplorEnz 1.4.1.13
PRIAM enzyme-specific profiles 1.4.1.13
KEGG Ligand Database for Enzyme Nomenclature 1.4.1.13
IUBMB Enzyme Nomenclature 1.4.1.13
IntEnz 1.4.1.13
MEDLINE Find literature relating to 1.4.1.13
MetaCyc 1.4.1.13
Rhea expert-curated reactions 1.4.1.13
UniProtKB/Swiss-Prot
O29309, AGLUS_ARCFU Q3Z7F6, AGLUS_DEHM1 Q58746, AGLUS_METJA
Q9WYM8, AGLUS_THEMA B9KBQ2, AGLUS_THENN B1L993, AGLUS_THESQ
P39812, GLTA_BACSU Q05755, GLTB_AZOBR O34399, GLTB_BACSU
P09831, GLTB_ECOLI E1V8I1, GLTB_HALED P96218, GLTB_MYCTU
Q05756, GLTD_AZOBR O08340, GLTD_CERSP P09832, GLTD_ECOLI
E1V8I0, GLTD_HALED P9WN18, GLTD_MYCTO P9WN19, GLTD_MYCTU

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