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ENZYME

ENZYME entry: EC 6.2.1.45

Accepted Name
E1 ubiquitin-activating enzyme
Alternative Name(s)
ubiquitin-activating enzyme E1
Reaction catalysed
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine <=> AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Comment(s)
  • Catalyzes the ATP-dependent activation of ubiquitin through the formation of a thioester bond between the C-terminal glycine of ubiquitin and the sulfhydryl side group of a cysteine residue in the E1 protein.
  • The two-step reaction consists of the ATP-dependent formation of an E1-ubiquitin adenylate intermediate in which the C-terminal glycine of ubiquitin is bound to AMP via an acyl-phosphate linkage, then followed by the conversion to an E1-ubiquitin thioester bond via the cysteine residue on E1 in the second step.
  • Formerly EC 6.3.2.19 and EC 6.3.2.21.
Cross-references
BRENDA 6.2.1.45
EC2PDB 6.2.1.45
ExplorEnz 6.2.1.45
PRIAM enzyme-specific profiles 6.2.1.45
KEGG Ligand Database for Enzyme Nomenclature 6.2.1.45
IUBMB Enzyme Nomenclature 6.2.1.45
IntEnz 6.2.1.45
MEDLINE Find literature relating to 6.2.1.45
MetaCyc 6.2.1.45
Rhea expert-curated reactions 6.2.1.45
UniProtKB/Swiss-Prot
P31254, UBA1Y_MOUSE A3KMV5, UBA1_BOVIN P52495, UBA1_CANAW
Q55C16, UBA1_DICDI P22314, UBA1_HUMAN Q02053, UBA1_MOUSE
Q29504, UBA1_RABIT Q5U300, UBA1_RAT O94609, UBA1_SCHPO
P22515, UBA1_YEAST A0AVT1, UBA6_HUMAN Q8C7R4, UBA6_MOUSE
P93028, UBE11_ARATH P20973, UBE11_WHEAT P92974, UBE12_ARATH
P31251, UBE12_WHEAT P31252, UBE13_WHEAT P31255, UBE1Y_OSPRU

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