Improving the positional accuracy of the goniometer on the Philips CM series TEM

J Pulokas; C Green; N Kisseberth; C S Potter; B Carragher

doi:10.1006/jsbi.1999.4181

Improving the positional accuracy of the goniometer on the Philips CM series TEM

J Struct Biol. 1999 Dec 30;128(3):250-6. doi: 10.1006/jsbi.1999.4181.

Authors

J Pulokas¹, C Green, N Kisseberth, C S Potter, B Carragher

Affiliation

¹ Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, Illinois, 61801, USA.

PMID: 10633064
DOI: 10.1006/jsbi.1999.4181

Abstract

We have developed a method to improve the accuracy for absolute relocation of a target specimen using the goniometer on a Philips transmission electron microscope. We have achieved this by characterizing the performance of the Philips compustage, modeling its behavior, and using this model to calculate the goniometer movements required for accurate target relocation. This resulted in a 10-fold improvement in the positioning accuracy of the goniometer.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Algorithms
Equipment Design / methods
Microscopy, Electron / instrumentation*
Reproducibility of Results