Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
Publication types
- Comparative Study
- Research Support, Non-U.S. Gov't
MeSH terms
- Coronavirus 229E, Human / genetics
- Coronavirus 229E, Human / isolation & purification
- Coronavirus Infections / diagnosis
- Coronavirus Infections / genetics
- Coronavirus Infections / virology*
- Coronavirus NL63, Human / genetics
- Coronavirus NL63, Human / isolation & purification
- Coronavirus OC43, Human / genetics
- Coronavirus OC43, Human / isolation & purification
- Humans
- Open Reading Frames
- Real-Time Polymerase Chain Reaction / methods*
- Saudi Arabia
- Sensitivity and Specificity
- Severe acute respiratory syndrome-related coronavirus / genetics*
- Severe acute respiratory syndrome-related coronavirus / isolation & purification*
- Travel
- Viral Envelope Proteins
- Viroporin Proteins
Substances
- E protein, SARS coronavirus
- Viral Envelope Proteins
- Viroporin Proteins