The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains

Virus Res. 1986 Aug;5(2-3):253-63. doi: 10.1016/0168-1702(86)90022-5.

Abstract

The amino acid sequence of the gene for the peplomer protein of the vaccine strain M41 and the Beaudette laboratory strain M42-Salk of avian infectious bronchitis virus (IBV) have been derived from cDNA sequences. As found with other coronaviruses, the peplomer protein carries the epitopes eliciting neutralizing antibodies. The gene encodes a primary translation product of 1162 amino acids with a molecular weight of 128,079. The use of a recent algorithm to predict membrane-protein interactions led to the unambiguous localization of the signal peptide and a transmembrane anchor alpha-helix at the C-terminus. At 50 positions amino acid differences were found between M41 and two Beaudette strains (M42-Salk and M42-Houghton). They are partly clustered in two regions of the protein. These two regions are candidates for neutralization epitopes of the protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Coronaviridae / genetics*
  • DNA / analysis
  • Epitopes / analysis
  • Genes*
  • Genes, Viral*
  • Infectious bronchitis virus / genetics*
  • Protein Conformation
  • Species Specificity
  • Viral Envelope Proteins / genetics*

Substances

  • Epitopes
  • Viral Envelope Proteins
  • DNA

Associated data

  • GENBANK/M21883