A novel closed-circular mitochondrial DNA with properties of a replicating intermediate

Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252-7. doi: 10.1073/pnas.68.9.2252.

Abstract

A fraction of the covalently closed mitochondrial DNA in mouse L cells contains a replicated heavy-strand segment that is hydrogen bonded to the circular light strand. The inserted single strand is dissociable from the circular duplex at an elevated temperature.

MeSH terms

  • Animals
  • Carbon Isotopes
  • Cell Fractionation
  • Centrifugation, Density Gradient
  • DNA / analysis*
  • DNA / isolation & purification
  • Hot Temperature
  • L Cells
  • Mice
  • Microscopy, Electron
  • Mitochondria / analysis*
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Protein Conformation*
  • Thymidine

Substances

  • Carbon Isotopes
  • DNA
  • Thymidine