Abstract
Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of sigma54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.
Publication types
- Research Support, U.S. Gov't, Non-P.H.S.
- Research Support, U.S. Gov't, P.H.S.
MeSH terms
- Amino Acid Sequence
- Bacillus subtilis / genetics*
- Base Sequence
- Cyanobacteria / genetics*
- DNA, Bacterial
- DNA-Binding Proteins*
- DNA-Directed RNA Polymerases / genetics*
- Genes, Bacterial
- Molecular Sequence Data
- Myxococcus xanthus / genetics*
- Phylogeny
- Polymerase Chain Reaction / methods*
- RNA Polymerase Sigma 54
- Saccharomyces cerevisiae / genetics
- Sigma Factor / genetics*
- Sinorhizobium meliloti / genetics*
- Trans-Activators / genetics*
Substances
- DNA, Bacterial
- DNA-Binding Proteins
- Sigma Factor
- Trans-Activators
- DNA-Directed RNA Polymerases
- RNA Polymerase Sigma 54