Highlights
- •
Adaption of coronaviruses OC43 and HKU1 to humans involved loss of HE lectin function
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OC43 HE receptor binding site was lost via progressive accumulation of mutations
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Loss of HE receptor binding alters sialate-9-O-acetylesterase receptor destroying activity
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Balance of receptor binding and receptor destruction contributes to host tropism
Summary
Graphical Abstract
Introduction
- de Groot R.J.
- Baker S.C.
- Baric R.
- Enjuanes L.
- Gorbalenya A.E.
- Holmes K.V.
- Perlman S.
- Poon L.
- Rottier P.J.M.
- Talbot P.J.
- et al.
- Lau S.K.P.
- Lee P.
- Tsang A.K.L.
- Yip C.C.Y.
- Tse H.
- Lee R.A.
- So L.-Y.
- Lau Y.-L.
- Chan K.-H.
- Woo P.C.Y.
- Yuen K.Y.
- de Groot R.J.
- Baker S.C.
- Baric R.
- Enjuanes L.
- Gorbalenya A.E.
- Holmes K.V.
- Perlman S.
- Poon L.
- Rottier P.J.M.
- Talbot P.J.
- et al.
- Lau S.K.P.
- Lee P.
- Tsang A.K.L.
- Yip C.C.Y.
- Tse H.
- Lee R.A.
- So L.-Y.
- Lau Y.-L.
- Chan K.-H.
- Woo P.C.Y.
- Yuen K.Y.
- de Groot R.J.
- Baker S.C.
- Baric R.
- Enjuanes L.
- Gorbalenya A.E.
- Holmes K.V.
- Perlman S.
- Poon L.
- Rottier P.J.M.
- Talbot P.J.
- et al.
- de Groot R.J.
- Baker S.C.
- Baric R.
- Enjuanes L.
- Gorbalenya A.E.
- Holmes K.V.
- Perlman S.
- Poon L.
- Rottier P.J.M.
- Talbot P.J.
- et al.
Results and Discussion
Lectin Properties of β1CoV HEs
Lectin Function of OC43 HE Impeded by a Combination of Mutations
Progressive Loss of HE Lectin Function during OC43 Evolution
Crystal Structure of an OC43A-type HE
Loss of HE Receptor Binding in HCoV-HKU1: A Case of Convergent Evolution?
- de Groot R.J.
- Baker S.C.
- Baric R.
- Enjuanes L.
- Gorbalenya A.E.
- Holmes K.V.
- Perlman S.
- Poon L.
- Rottier P.J.M.
- Talbot P.J.
- et al.
- Huang X.
- Dong W.
- Milewska A.
- Golda A.
- Qi Y.
- Zhu Q.K.
- Marasco W.A.
- Baric R.S.
- Sims A.C.
- Pyrc K.
- et al.
Loss of HE Lectin Function Reduces Receptor-Destroying Enzyme Activity toward Multivalent Substrates
Consequences of Loss of Lectin Function for Virion-Mediated Receptor Destruction
Conclusion
Experimental Procedures
Expression and Purification of HEs
Purification of Virions for Whole-Virus Receptor-Destruction Assays
Hemagglutination Assay
Solid-Phase Lectin-Binding Assay and On-the-Plate O-Ac-Sia Depletion Assay
Author Contributions
Acknowledgments
Accession Numbers
Supplemental Information
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Document S1. Supplemental Experimental Procedures, Figures S1–S5, and Tables S1 and S2
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