Summary
A micro-color test is described in which destruction of monolayers of secondary pig thyroid cells in disposable plastic trays as visualized macroscopically after staining with crystal violet is used as an indicator of viral multiplication. This test proved to be sensitive and to give reproducible results when applied to the titration of transmissible gastroenteritis (TGE) virus-neutralizing antibodies. It was, however, considerable less sensitive than a macro procedure when used for the titration of TGE virus.
Similar content being viewed by others
References
Harada, K., T. Kumagai, andJ. Sasahara: Cytopathogenicity of transmissible gastroenteritis virus in pigs. Nat. Inst. anim. Hlth Quart.3, 166–167 (1963).
Cartwright, Sheila F., Helen M. Harris, T. B. Blandford, I. Fincham, andM. Gitter: Transmissible gastro-enteritis of pigs. Vet. Rec.76, 1332–1333 (1964).
Bohl, E. H., and T.Kumagai: The use of cell cultures for the study of TGE virus of swine. Proc. 69th annual meeting U.S. Livestock San. Ass. 343–350 (1965).
McClurkin, A. W.: Studies on transmissible gastroenteritis of swine. I. The isolation and identification of a cytopathogenic virus of transmissible gastroenteritis in primary swine kidney cell cultures. Canad. J. comp. Med.29, 46–53 (1965).
Sheffy, B. E.: Characterization of transmissible gastroenteritis virus. Proc. 69th annual meeting U.S. Livestock San. Ass. 351–360 (1965).
Cartwright, Sheila F.: A cytopathic virus causing a transmissible gastroenteritis in swine. II. Biological and serological studies. J. comp. Path.76, 95–106 (1966).
McClurkin, A. W., andJ. O. Norman: Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Canad. J. comp. Med.30, 190–198 (1966).
Bohl, E. H.: Immunologic studies on transmissible gastroenteritis of swine. Proc. 18th World vet. Congr.2, 577 (1967).
Harada, K., T. Kumagai, andJ. Sasahara: Studies on transmissible gastroenteritis in pigs. III. Isolation of cytopathogenic virus and its use for serological investigation. Nat. Inst. anim. Hlth Quart.7, 127–137 (1967).
Derbyshire, J. B., D. M. Jessett, andG. Newman: An experimental epidemiological study of porcine transmissible gastroenteritis. J. comp. Path.79, 445–452 (1969).
Harada, K., S. Furuuchi, T. Kumagai, andJ. Sasahara: Pathogenicity, immunogenicity and distribution of transmissible gastroenteritis virus in pigs. Nat. Inst. anim. Hlth Quart.9, 185–192 (1969).
Witte, K. H., andB. C. Easterdat: Isolation and propagation of the virus of transmissible gastroenteritis of pigs in various pig cell cultures. Arch. ges. Virusforsch.20, 327–350 (1967).
Witte, K. H., andB. C. Easterday: Stained monolayer test: a color test in disposable plastic trays for titrating transmissible gastroenteritis virus and neutralizing antibodies. Amer. J. vet. Res.29, 1409–1417 (1968).
Liebermann, H.: Die serologische Diagnose der Transmissiblen Gastroenteritis der Schweine. Arch. exp. Vet.-Med.23, 811–826 (1969).
Takátsy, G.: A new method for the preparation of serial dilutions in a quick and accurate way. Kisérl. orvostudom.2, 393–396 (1950).
Sever, J. L.: Application of a microtechnique to viral serological investigations. J. Immunol.88, 320–329 (1962).
Dougherty, R. M.: Animal virus titration techniques. In: Techniques in Experimental Virology (R. J. C., Harris, ed.), 183–196. New York: Academic Press, 1964.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Witte, K.H. Micro-color test for assay of transmissible gastroenteritis virus-neutralizing antibodies. Archiv f Virusforschung 33, 171–176 (1971). https://doi.org/10.1007/BF01254174
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01254174