A total of 508 unrelated males from the North Poland population were analyzed for 9 Y-chromosome STRs (DYS 19, DYS 390, DYS 393, DYS 392, DYS 391, DYS 389I, DYS 389II and DYS 385I/II) using two multiplex reactions and detection of PCR products using capillary electrophoresis. In the analyzed sample 328 different haplotypes were identified, among which 264 were unique. It was found that the model for a Polish population haplotype (DYS 19*17, DYS 390*25, DYS 393*13, DYS 392*11, DYS 391*10, DYS 389I*13, DYS 389II*30, DYS 385I/II*10,14) is almost 15 times more frequent in our population than in a cumulative European one. The haplotype diversity/discrimination index calculated for 9 loci is 0.9943. In the analysed population sample three mutations were detected in the DYS19 (duplication) and DYS385I/II loci (triplications).