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Perioperative Mitigation of Oxidative Stress with Molecular Hydrogen During Simulated Heart Transplantation in Pigs

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Molecular Hydrogen in Health and Disease

Part of the book series: Advances in Biochemistry in Health and Disease ((ABHD,volume 27))

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Abstract

Heart transplantation is now a routine method for severe heart failure treatment. It is critical to focus on preventing ischemia–reperfusion damage and mitigating oxidative stress to achieve successful outcomes. However, prolonged anesthesia, hyperoxia, and defibrillations contribute to an increase of ROS/RNS and disrupt the redox homeostasis, which poses a serious risk factor. Numerous publications have confirmed the remarkable antioxidant, anti-apoptotic, and anti-inflammatory properties of molecular hydrogen. In our simulated heart transplantation experiment, we demonstrate that administering 2% hydrogen gas during anesthesia and extracorporeal circulation (ECC) significantly alleviates oxidative stress-induced damage. This is evidenced by a significant decrease in markers of ischemia, lipid peroxidation, and inflammation. The restoration of the pumping activity in the implanted pig hearts showed improvement, with a reduced need for repeated defibrillations. The administration of H2 during graft collection and transplantation significantly enhances the function of the transplanted heart and the overall condition of the recipient. Hydrogen administered by conventional ventilators and ECC oxygenators represents an innovative therapy that can significantly improve current transplantation techniques.

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Competing Interest

We thank the management of IKEM Prague for hiring operating rooms and transplant assistants. We thank the collaborating team of the Slovak Institute of Heart Diseases (Drs. M. Hulman, V. Hudec, J. Luptak, I-Olejarova, M. Ondrusek, I. Gasparovic, R.Sramaty) for performing simulated transplantation.

Funding

This research was funded by grants from Slovak Research and Development Agency (APVV-0241–11, APVV-15–0376, APVV-19–0317), grant from the Slovak Academy of Sciences (VEGA 2/0092/22 and 2/0063/18), grant from European Union Structural funds (ITMS 26230120009), grant (2018/7838:1-26C0), and grant from Ministry of Health of The Slovak Republic (2019-CEMSAV-1). Authors declare no conflict of interests.

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Authors and Affiliations

  1. Centre of Experimental Medicine, Institute for Heart Research, Slovak Academy of Sciences, Dúbravská Cesta 9, Bratislava, 84104, Slovak Republic

    Branislav Kura, Barbara Szeiffova Bacova, Miroslav Barancik, Matus Sykora, Ludmila Okruhlicova, Narcisa Tribulova, Barbora Kalocayova, Katarina Andelova & Jan Slezak

  2. Institute of Molecular Cardiology, University of Louisville, Louisville, KY, 40202, USA

    Roberto Bolli

  3. Molecular Hydrogen Institute, Enoch, UT, 84721, USA

    Tyler W. LeBaron

  4. Department of Kinesiology and Outdoor Recreation, Southern Utah University, Cedar City, UT, 84720, USA

    Tyler W. LeBaron

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  1. Branislav Kura
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  2. Barbara Szeiffova Bacova
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  11. Jan Slezak
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Correspondence to Jan Slezak .

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Editors and Affiliations

  1. Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia

    Jan Slezak

  2. Centre of Experimental Medicine, Slovak Academy of Sciences, Bratislava, Slovakia

    Branislav Kura

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Kura, B. et al. (2024). Perioperative Mitigation of Oxidative Stress with Molecular Hydrogen During Simulated Heart Transplantation in Pigs. In: Slezak, J., Kura, B. (eds) Molecular Hydrogen in Health and Disease. Advances in Biochemistry in Health and Disease, vol 27. Springer, Cham. https://doi.org/10.1007/978-3-031-47375-3_12

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