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Spontaneous Protein Adsorption on Graphene Oxide Nanosheets Allowing Efficient Intracellular Vaccine Protein Delivery

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† Department of Pharmaceutics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
‡ VIB Inflammation Research Center, Ghent University, Technologiepark 927, 9052 Zwijnaarde, Belgium
§ Department of Respiratory Medicine, University Hospital Ghent, De Pintelaan 185, 9000 Ghent, Belgium
∥ Institute for Bioengineering of Catalonia, Carrer de Baldiri Reixac, 10, 08028 Barcelona, Spain
⊥ Department of Biomedical Molecular Biology, Ghent University, Technologiepark 927, 9052 Zwijnaarde, Belgium
# Department of Pharmaceutical Analysis, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium
*Tel: +32 9 264 80 55. E-mail: [email protected]
Cite this: ACS Appl. Mater. Interfaces 2016, 8, 2, 1147–1155
Publication Date (Web):December 22, 2015
https://doi.org/10.1021/acsami.5b08963
Copyright © 2015 American Chemical Society
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    Nanomaterials hold potential of altering the interaction between therapeutic molecules and target cells or tissues. High aspect ratio nanomaterials in particular have been reported to possess unprecedented properties and are intensively investigated for their interaction with biological systems. Graphene oxide (GOx) is a water-soluble graphene derivative that combines high aspect ratio dimension with functional groups that can be exploited for bioconjugation. Here, we demonstrate that GOx nanosheets can spontaneously adsorb proteins by a combination of interactions. This property is then explored for intracellular protein vaccine delivery, in view of the potential of GOx nanosheets to destabilize lipid membranes such as those of intracellular vesicles. Using a series of in vitro experiments, we show that GOx nanosheet adsorbed proteins are efficiently internalized by dendritic cells (DCs: the most potent class of antigen presenting cells of the immune system) and promote antigen cross-presentation to CD8 T cells. The latter is a hallmark in the induction of potent cellular antigen-specific immune responses against intracellular pathogens and cancer.

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