CD14, a Receptor for Complexes of Lipopolysaccharide (LPS) and LPS Binding Protein
Abstract
Leukocytes respond to lipopolysaccharide (LPS) at nanogram per milliliter concentrations with secretion of cytokines such as tumor necrosis factor-α (TNF-α). Excess secretion of TNF-α causes endotoxic shock, an often fatal complication of infection. LPS in the bloodstream rapidly binds to the serum protein, lipopolysaccharide binding protein (LBP), and cellular responses to physiological levels of LPS are dependent on LBP. CD14, a differentiation antigen of monocytes, was found to bind complexes of LPS and LBP, and blockade of CD14 with monoclonal antibodies prevented synthesis of TNF-α by whole blood incubated with LPS. Thus, LPS may induce responses by interacting with a soluble binding protein in serum that then binds the cell surface protein CD14.
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References
ASHMUN, R.A., EXPRESSION OF THE HUMAN MONOCYTE MEMBRANE ANTIGEN GP55 BY MURINE FIBROBLASTS AFTER DNA-MEDIATED GENE-TRANSFER, BLOOD 69: 886 (1987).
BARNSTABLE, C.J., PRODUCTION OF MONOCLONAL ANTIBODIES TO GROUP-A ERYTHROCYTES, HLA AND OTHER HUMAN CELL-SURFACE ANTIGENS - NEW TOOLS FOR GENETIC-ANALYSIS, CELL 14: 9 (1978).
DESCH, C.E., PRODUCTION OF HUMAN-TUMOR NECROSIS FACTOR FROM WHOLE-BLOOD EXVIVO, LYMPHOKINE RESEARCH 8: 141 (1989).
ESPEVIK, T, A HIGHLY SENSITIVE CELL-LINE, WEHI-164 CLONE 13, FOR MEASURING CYTOTOXIC FACTOR TUMOR-NECROSIS-FACTOR FROM HUMAN-MONOCYTES, JOURNAL OF IMMUNOLOGICAL METHODS 95: 99 (1986).
FERRERO, E, NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE MONOCYTE DIFFERENTIATION ANTIGEN, CD14, NUCLEIC ACIDS RESEARCH 16: 4173 (1988).
Fleit, H. B., Proceedings of the National Academy of Sciences of the United States of America 79: 3275 (1982).
GOYERT, S.M., THE CD14 MONOCYTE DIFFERENTIATION ANTIGEN MAPS TO A REGION ENCODING GROWTH-FACTORS AND RECEPTORS, SCIENCE 239: 497 (1988).
HAZIOT, A, THE MONOCYTE DIFFERENTIATION ANTIGEN, CD14, IS ANCHORED TO THE CELL-MEMBRANE BY A PHOSPHATIDYLINOSITOL LINKAGE, JOURNAL OF IMMUNOLOGY 141: 547 (1988).
KUDRYK, B, SPECIFICITY OF A MONOCLONAL-ANTIBODY FOR THE NH2-TERMINAL REGION OF FIBRIN, MOLECULAR IMMUNOLOGY 21: 89 (1984).
MCFARLAND, K.C., LUTROPIN-CHORIOGONADOTROPIN RECEPTOR - AN UNUSUAL MEMBER OF THE G-PROTEIN COUPLED RECEPTOR FAMILY, SCIENCE 245: 494 (1989).
MICHIE, H.R., DETECTION OF CIRCULATING TUMOR NECROSIS FACTOR AFTER ENDOTOXIN ADMINISTRATION, NEW ENGLAND JOURNAL OF MEDICINE 318: 1481 (1988).
MICHL, J, EFFECTS OF IMMOBILIZED IMMUNE-COMPLEXES ON FC-RECEPTOR AND COMPLEMENT-RECEPTOR FUNCTION IN RESIDENT AND THIOGLYCOLLATE-ELICITED MOUSE PERITONEAL-MACROPHAGES, JOURNAL OF EXPERIMENTAL MEDICINE 150: 607 (1979).
SCHUMANN, R.R., STRUCTURE AND FUNCTION OF LIPOPOLYSACCHARIDE BINDING-PROTEIN, SCIENCE 249: 1429 (1990).
SETOGUCHI, M, MOUSE AND HUMAN CD14 (MYELOID CELL-SPECIFIC LEUCINE-RICH GLYCOPROTEIN) PRIMARY STRUCTURE DEDUCED FROM CDNA CLONES, BIOCHIMICA ET BIOPHYSICA ACTA 1008: 213 (1989).
SHERRY, B, CACHECTIN TUMOR NECROSIS FACTOR EXERTS ENDOCRINE, PARACRINE, AND AUTOCRINE CONTROL OF INFLAMMATORY RESPONSES, JOURNAL OF CELL BIOLOGY 107: 1269 (1988).
SIMMONS, D.L., MONOCYTE ANTIGEN CD14 IS A PHOSPHOLIPID ANCHORED MEMBRANE-PROTEIN, BLOOD 73: 284 (1989).
TOBIAS, P.S., IDENTIFICATION OF A LIPID-A BINDING-SITE IN THE ACUTE PHASE REACTANT LIPOPOLYSACCHARIDE BINDING-PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 10867 (1989).
TOBIAS, P.S., ISOLATION OF A LIPOPOLYSACCHARIDE-BINDING ACUTE PHASE REACTANT FROM RABBIT SERUM, JOURNAL OF EXPERIMENTAL MEDICINE 164: 777 (1986).
TODD, R.F., STRUCTURAL-ANALYSIS OF DIFFERENTIATION ANTIGENS MO1 AND MO2 ON HUMAN-MONOCYTES, HYBRIDOMA 1: 329 (1982).
ULEVITCH, R.J., unpublished data.
VANVOORHIS, W.C., SPECIFIC ANTIMONONUCLEAR PHAGOCYTE MONOCLONAL-ANTIBODIES - APPLICATION TO THE PURIFICATION OF DENDRITIC CELLS AND THE TISSUE LOCALIZATION OF MACROPHAGES, JOURNAL OF EXPERIMENTAL MEDICINE 158: 126 (1983).
WRIGHT, S.D., TUMOR-PROMOTING PHORBOL ESTERS STIMULATE C3B AND C3B' RECEPTOR-MEDIATED PHAGOCYTOSIS IN CULTURED HUMAN-MONOCYTES, JOURNAL OF EXPERIMENTAL MEDICINE 156: 1149 (1982).
WRIGHT, S.D., LIPOPOLYSACCHARIDE (LPS) BINDING-PROTEIN OPSONIZES LPS-BEARING PARTICLES FOR RECOGNITION BY A NOVEL RECEPTOR ON MACROPHAGES, JOURNAL OF EXPERIMENTAL MEDICINE 170: 1231 (1989).
WRIGHT, S.D., CD18-DEFICIENT CELLS RESPOND TO LIPOPOLYSACCHARIDE INVITRO, JOURNAL OF IMMUNOLOGY 144: 2566 (1990).
WRIGHT, S.D., METHODS IN ENZYMOLOGY 132: 204 (1986).
Wright, S. D., Proceedings of the National Academy of Sciences of the United States of America 80: 5699 (1983).
WRIGHT, S.D., unpublished data.
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Science
Volume 249 | Issue 4975
21 September 1990
21 September 1990
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- Samuel D. Wright et al.
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