Abstract
Testing for neutralizing antibodies against polioviruses has been an established gold standard for assessing individual protection from disease, population immunity, vaccine efficacy studies, and other vaccine clinical trials. Detecting poliovirus specific IgM and IgA in sera and mucosal specimens has been proposed for evaluating the status of population mucosal immunity. More recently, there has been a renewed interest in using dried blood spot cards as a medium for sample collection to enhance surveillance of poliovirus immunity. Here, we describe the modified poliovirus microneutralization assay, poliovirus capture IgM and IgA ELISA assays, and dried blood spot polio serology procedures for the detection of antibodies against poliovirus serotypes 1, 2, and 3.
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© 2016 Springer Science+Business Media New York
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Weldon, W.C., Oberste, M.S., Pallansch, M.A. (2016). Standardized Methods for Detection of Poliovirus Antibodies. In: Martín, J. (eds) Poliovirus. Methods in Molecular Biology, vol 1387. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3292-4_8
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DOI: https://doi.org/10.1007/978-1-4939-3292-4_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3291-7
Online ISBN: 978-1-4939-3292-4
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