A Simple and General Method for Transferring Genes into Plants
Abstract
Transformed petunia, tobacco, and tomato plants have been produced by means of a novel leaf disk transformation-regeneration method. Surface-sterilized leaf disks were inoculated with an Agrobacterium tumefaciens strain containing a modified tumor-inducing plasmid (in which the phytohormone biosynthetic genes from transferred DNA had been deleted and replaced with a chimeric gene for kanamycin resistance) and cultured for 2 days. The leaf disks were then transferred to selective medium containing kanamycin. Shoot regeneration occurred within 2 to 4 weeks, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.
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Published In
Science
Volume 227 | Issue 4691
8 March 1985
8 March 1985
Copyright
1985 by the American Association for the Advancement of Science.
Submission history
Received: 1 October 1984
Accepted: 19 November 1984
Published in print: 8 March 1985
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- R. B. Horsch et al.
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