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Interaction of Apoplastic Peroxidases from Wheat Roots with Nitrite and Nitrate: Intermediates and Products

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Abstract

Peroxidases are widespread in animal and plant tissues, wherein they perform a variety of functions. Peroxidases have a broad specificity for substrates of various chemical structures. Along with hydrogen peroxide, phenolic compounds, and toxic compounds of aromatic nature, nitrogen-containing compounds are substrates for peroxidases. This work is devoted to the study of the role of wheat extracellular peroxidases in the metabolism of nitrogen-containing compounds. It has been shown that partially purified isozymes differing in peroxidase activity are involved in the metabolism of nitrogen-containing compounds. The formation of primary and secondary phenoxyl radicals during the combined oxidation of chlorogenic acid, nitrite, and H2O2 was demonstrated. With cooxidation with purified isoenzymes p˗coumaric acid and nitrite, the formation of 4˗hydroxy˗3˗nitrocinnamic acid was revealed. It is assumed that the same isoforms can participate both in the oxidation of nitrite with the formation of nitrophenol and in the reduction of nitrate. The participation of plant peroxidases in nitrogen metabolism can be represented as a set of reactions for the reduction and/or oxidation of nitrogen of different oxidation states with the formation of active intermediates.

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Funding

The work was carried out within the framework of the state assignment of the Federal Research Center Kazan Scientific Center of the Russian Academy of Sciences (analysis of enzyme activity) using the equipment of the Collective Spectro-Analytical Center for Physical and Chemical Research of the Structure, Composition and Properties of Substances and Materials at the Kazan Scientific Center of the Russian Academy of Sciences and partly has been supported by the Kazan Federal University Strategic Academic Leadership Program (obtaining a chemical standard).

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    E. I. Galeeva, L. V. Viktorova, O. P. Guryanov, A. V. Chasov & F. V. Minibayeva

  2. Zavoisky Physical-Technical Institute, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    A. A. Sukhanov

  3. Kazan (Volga Region) Federal University, Kazan, Russia

    A. V. Chasov & F. V. Minibayeva

Authors
  1. E. I. Galeeva
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  2. L. V. Viktorova
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  3. O. P. Guryanov
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  4. A. A. Sukhanov
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  5. A. V. Chasov
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  6. F. V. Minibayeva
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Correspondence to E. I. Galeeva or F. V. Minibayeva.

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Conflict of interests. The authors declare that they have no conflicts of interest.

Statement on the welfare of humans or animals. This article does not contain any studies involving humans or animals performed by any of the authors.

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Abbreviations: ROS—reactive oxygen species; RNS—reactive nitrogen species; POX—peroxidase; ECS—extracellular solution.

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Galeeva, E.I., Viktorova, L.V., Guryanov, O.P. et al. Interaction of Apoplastic Peroxidases from Wheat Roots with Nitrite and Nitrate: Intermediates and Products. Russ J Plant Physiol 69, 8 (2022). https://doi.org/10.1134/S1021443722010046

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  • DOI: https://doi.org/10.1134/S1021443722010046

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