Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19

Simon P Graham; Rebecca K McLean; Alexandra J Spencer; Sandra Belij-Rammerstorfer; Daniel Wright; Marta Ulaszewska; Jane C Edwards; Jack W P Hayes; Veronica Martini; Nazia Thakur; Carina Conceicao; Isabelle Dietrich; Holly Shelton; Ryan Waters; Anna Ludi; Ginette Wilsden; Clare Browning; Dagmara Bialy; Sushant Bhat; Phoebe Stevenson-Leggett; Philippa Hollinghurst; Ciaran Gilbride; David Pulido; Katy Moffat; Hannah Sharpe; Elizabeth Allen; Valerie Mioulet; Chris Chiu; Joseph Newman; Amin S Asfor; Alison Burman; Sylvia Crossley; Jiandong Huo; Raymond J Owens; Miles Carroll; John A Hammond; Elma Tchilian; Dalan Bailey; Bryan Charleston; Sarah C Gilbert; Tobias J Tuthill; Teresa Lambe

doi:10.1038/s41541-020-00221-3

Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19

NPJ Vaccines. 2020 Jul 27;5(1):69. doi: 10.1038/s41541-020-00221-3. eCollection 2020.

Authors

Simon P Graham¹, Rebecca K McLean^#¹, Alexandra J Spencer^#², Sandra Belij-Rammerstorfer², Daniel Wright², Marta Ulaszewska², Jane C Edwards¹, Jack W P Hayes¹, Veronica Martini¹, Nazia Thakur¹, Carina Conceicao¹, Isabelle Dietrich¹, Holly Shelton¹, Ryan Waters¹, Anna Ludi¹, Ginette Wilsden¹, Clare Browning¹, Dagmara Bialy¹, Sushant Bhat¹, Phoebe Stevenson-Leggett¹, Philippa Hollinghurst^{1 3}, Ciaran Gilbride², David Pulido², Katy Moffat¹, Hannah Sharpe², Elizabeth Allen², Valerie Mioulet¹, Chris Chiu¹, Joseph Newman¹, Amin S Asfor¹, Alison Burman¹, Sylvia Crossley¹, Jiandong Huo^{4 5}, Raymond J Owens^{4 5}, Miles Carroll⁶, John A Hammond¹, Elma Tchilian¹, Dalan Bailey¹, Bryan Charleston¹, Sarah C Gilbert², Tobias J Tuthill^#¹, Teresa Lambe^#²

Affiliations

¹ The Pirbright Institute, Ash Road, Pirbright, GU24 0NF UK.
² The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ UK.
³ Department of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, GU2 7XH UK.
⁴ Protein Production UK, Research Complex at Harwell, and Rosalind Franklin Institute Rutherford Appleton Laboratory Harwell Oxford, Didcot, OX11 0FA UK.
⁵ Division of Structural Biology, Henry Wellcome Building for Genomic Medicine, University of Oxford, Oxford, OX3 7BN UK.
⁶ Public Health England, Manor Farm Road, Salisbury, SP4 0JG UK.

^# Contributed equally.

Abstract

Clinical development of the COVID-19 vaccine candidate ChAdOx1 nCoV-19, a replication-deficient simian adenoviral vector expressing the full-length SARS-CoV-2 spike (S) protein was initiated in April 2020 following non-human primate studies using a single immunisation. Here, we compared the immunogenicity of one or two doses of ChAdOx1 nCoV-19 in both mice and pigs. Whilst a single dose induced antigen-specific antibody and T cells responses, a booster immunisation enhanced antibody responses, particularly in pigs, with a significant increase in SARS-CoV-2 neutralising titres.

Keywords: Vaccines; Virology.

Abstract

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