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Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions

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Abstract

The kinetics of cysteine and divalent ion modulation viz. Ca2+, Cu2+, Hg2+ of fruit bromelain (EC 3.4.22.33) have been investigated in the present study. Kinetic studies revealed that at pH 4.5, cysteine induced V-type activation of bromelain catalyzed gelatin hydrolysis. At pH 3.5, Ca2+ inhibited the enzyme noncompetitively, whereas, both K-and V-type activations of bromelain were observed in the presence of 0.5 mM Ca2+ at pH 4.5 and 7.5. Bromelain was inhibited competitively at 0.6 mM Cu2+ ions at pH 3.5, which changed to an uncompetitive inhibition at pH 4.5 and 7.5. An un-competitive inhibition of bromelain catalyzed gelatin hydrolysis was observed in the presence of 0.6 mM Hg2+ at pH 3.5 and 4.5. These findings suggest that divalent ions modulation of fruit bromelain is pH dependent.

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Acknowledgments

The authors are grateful to the management of Lovely Professional University, Phagwara (India) for providing facilities to conduct the laboratory work.

Conflict of interest

There is no conflict of interest among authors of this publication.

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Authors and Affiliations

  1. Department of Biochemistry, School of Biotechnology and Biosciences, Lovely Professional University, 144401, Phagwara, Punjab, India

    Tajwinder Kaur, Amandeep Kaur & Ravneet K Grewal

Authors
  1. Tajwinder Kaur
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  2. Amandeep Kaur
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  3. Ravneet K Grewal
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Correspondence to Ravneet K Grewal.

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Research highlights

1. We examined the kinetic behavior of fruit bromelain in the presence of activators i.e. cysteine and Ca2+, and inhibitors i.e. Cu2+ and Hg2+.

2. Bromelain activity is enhanced with V-type activation in the presence of cysteine, however, both K and V-type activations are observed in the presence of Ca2+ ions.

3. Cu2+ and Hg2+ ions inhibit the enzyme uncompetitively.

4. We examined the effects of divalent ions i.e. Ca2+, Cu2+ and Hg2+ on kinetic parameters of bromelain as the function of pH.

5. Metal ion interactions with bromelain from Ananas comosus are pH dependent.

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Kaur, T., Kaur, A. & Grewal, R.K. Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions. J Food Sci Technol 52, 5954–5960 (2015). https://doi.org/10.1007/s13197-014-1639-5

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  • DOI: https://doi.org/10.1007/s13197-014-1639-5

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