Abstract
To combat severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and any unknown emerging pathogens in the future, the development of a rapid and effective method to generate high-affinity antibodies or antibody-like proteins is of critical importance. We here report high-speed in vitro selection of multiple high-affinity antibody-like proteins against various targets including the SARS-CoV-2 spike protein. The sequences of monobodies against the SARS-CoV-2 spike protein were successfully procured within only 4 days. Furthermore, the obtained monobody efficiently captured SARS-CoV-2 particles from the nasal swab samples of patients and exhibited a high neutralizing activity against SARS-CoV-2 infection (half-maximal inhibitory concentration, 0.5 nanomolar). High-speed in vitro selection of antibody-like proteins is a promising method for rapid development of a detection method for, and of a neutralizing protein against, a virus responsible for an ongoing, and possibly a future, pandemic.
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Amino Acid Sequence
- Angiotensin-Converting Enzyme 2
- Antibodies, Immobilized / chemistry
- Antibodies, Immobilized / immunology
- Antibodies, Neutralizing / chemistry
- Antibodies, Neutralizing / immunology
- Antibodies, Neutralizing / metabolism
- Betacoronavirus / genetics
- Betacoronavirus / immunology*
- Betacoronavirus / isolation & purification
- COVID-19
- Cell Surface Display Techniques / methods
- Coronavirus Infections / pathology
- Coronavirus Infections / virology
- Dimerization
- Humans
- Kinetics
- Pandemics
- Peptides / chemistry
- Peptides / immunology
- Peptidyl-Dipeptidase A / chemistry
- Peptidyl-Dipeptidase A / immunology*
- Peptidyl-Dipeptidase A / metabolism
- Pneumonia, Viral / pathology
- Pneumonia, Viral / virology
- Protein Domains / immunology
- Protein Subunits / chemistry
- Protein Subunits / immunology
- Protein Subunits / metabolism
- RNA, Viral / metabolism
- SARS-CoV-2
- Single-Domain Antibodies / chemistry
- Single-Domain Antibodies / immunology*
- Single-Domain Antibodies / metabolism
- Spike Glycoprotein, Coronavirus / chemistry
- Spike Glycoprotein, Coronavirus / immunology*
Substances
- Antibodies, Immobilized
- Antibodies, Neutralizing
- Peptides
- Protein Subunits
- RNA, Viral
- Single-Domain Antibodies
- Spike Glycoprotein, Coronavirus
- spike protein, SARS-CoV-2
- Peptidyl-Dipeptidase A
- ACE2 protein, human
- Angiotensin-Converting Enzyme 2