Journal of Medical Virology

Volume 87, Issue 7 p. 1096-1103

Research Article

Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549

Liqi Wu,

Liqi Wu

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

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Jianping Dai,

Jianping Dai

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

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Xiangfeng Zhao,

Xiangfeng Zhao

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

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Youying Chen,

Youying Chen

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

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Gefei Wang,

Gefei Wang

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

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Kangsheng Li,

Corresponding Author

Kangsheng Li

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Correspondence to: Kangsheng Li, MD, PhD, Department of Microbiology and Immunology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, China.

E-mail: [email protected]

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Liqi Wu,

Liqi Wu

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Search for more papers by this author

Jianping Dai,

Jianping Dai

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Search for more papers by this author

Xiangfeng Zhao,

Xiangfeng Zhao

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Search for more papers by this author

Youying Chen,

Youying Chen

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Search for more papers by this author

Gefei Wang,

Gefei Wang

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Search for more papers by this author

Kangsheng Li,

Corresponding Author

Kangsheng Li

Department of Microbiology and Immunology, Shantou University Medical College, Shantou, China

The Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, Shantou, China

Correspondence to: Kangsheng Li, MD, PhD, Department of Microbiology and Immunology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, China.

E-mail: [email protected]

Search for more papers by this author

First published: 25 February 2015

https://doi.org/10.1002/jmv.24135

Citations: 12

None of the authors has any conflict of interest to declare.

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Abstract

Anti-malaria drug, chloroquine, has been reported to be effective against influenza A virus (IAV) in vitro and used in in-vivo experiments and clinical trial for prevention or treatment of influenza. In this study, it has been shown by immunofluorescence, hemagglutination, and plaque assays that chloroquine enhanced A/WSN/33 (H1N1) replication with pronounced cytopathic effect in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549. Time-of-addition assay showed that inhibitory effect on virus replication by chloroquine pre-treatment was indistinctive, and virus productions were enhanced when the drug was applied after viral adsorption. The effectiveness of chloroquine as an anti-influenza drug is questioned, and caution in its use is recommended. J. Med. Virol. 87:1096–1103, 2015. © 2015 Wiley Periodicals, Inc.

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Citing Literature

Volume87, Issue7

July 2015

Pages 1096-1103

Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549

Abstract

Supporting Information

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Citing Literature

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Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549

Abstract

Supporting Information

REFERENCES

Citing Literature

References

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