Abstract
A clonal propagation method has been developed for efficient multiplication ofVanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l−1) and α-naphthaleneacetic acid (NAA, 1 mg l−1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l−1 and NAA at 0.5 mg l−1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots inV. planifolia.
Similar content being viewed by others
Abbreviations
- BA :
-
N6-benzyladenine
- DMRT :
-
Duncan's multiple-range test
- KC :
-
Knudson (1946) medium
- KCB :
-
KC basal medium
- Kn :
-
kinetin
- MS :
-
Murashige and Skoog (1962) medium
- MSB :
-
MS basal medium
- 1/2 MSB :
-
half-strength MSB
- MS-D :
-
double-phase MS medium
- MS-L :
-
liquid MS medium
- MS-S :
-
semi-solid MS medium
- NAA :
-
α-Naphthaleneacetic acid
References
Borkird C, Sink KC (1983) Medium components for shoot cultures of chlorophyll-deficient mutants ofPetunia inflata. Plant Cell Rep 2:1–4
Davidonis G, Knorr D (1991) Callus formation and shoot regeneration inVanilla planifolia. Food Biotechnol 5:59–66
Duncan DB (1955) Multiple range and multipleF test. Biometrics 11:1–42
Goodenough DR (1982)Vanilla, vanillin and vanillin derivatives. Bakers Dig 56:8–10
Gu Z, Arditti J, Nyman LP (1987)Vanilla planifolia: callus induction and plantlet production in vitro. Lindleyana 2:48–52
Knorr D, Caster C, Dorneburg H, Dorn R, Graf S, Havkin-Frenkel D, Podstolski A, Werrman U (1993) Biosynthesis and yield improvement of food ingredients from plant cell and tissue cultures. Food Technol 12:57–63
Knudson L (1946) A new nutrient solution for the germination of orchid seed. Am Orchid Soc Bull 15:214–217
Kononowicz H, Janick J (1984) In vitro propagation ofVanilla planifolia. Hort Science 19:58–59
Krikorian AD, Kann RP (1979) Clonal propagation of daylilies. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture. Ohio State University Press, Columbus, pp 835–836
Molnar GY (1987) A new patented method for mass propagation of shoot cultures. Acta Hort 212:125–130
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Philip VJ, Nainar SAZ (1986) Clonal propagation ofVanilla planifolia (Salisb) Ames using tissue culture. J Plant Physiol 122:211–215
Simmonds JA, Cumming BG (1976) Propagation ofLilium hybrids. II. Production of plantlets from bulb scale callus cultures for increased propagation rates. Sci Hort 5:161–170
Stafford A (1991) The manufacture of food ingredients using plant cell and tissue cultures. Trends Food Sci Technol 2:116–122
Author information
Authors and Affiliations
Additional information
Communicated by G. C. Phillips
Rights and permissions
About this article
Cite this article
George, P.S., Ravishankar, G.A. In vitro multiplication ofVanilla planifolia using axillary bud explants. Plant Cell Reports 16, 490–494 (1997). https://doi.org/10.1007/BF01092772
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01092772