Elsevier

Clinica Chimica Acta

Volume 510, November 2020, Pages 35-46
Clinica Chimica Acta

Review
Laboratory diagnosis of coronavirus disease-2019 (COVID-19)

https://doi.org/10.1016/j.cca.2020.06.045 Get rights and content

Highlights

  • We summarized the existing laboratory testing methods for SARS-CoV-2, including nucleic acid tests, antibody tests and genomic sequencing.

  • We compared the advantages and disadvantages of different laboratory testing methods for COVID-19, and provided an outlook of the improvement and development of detection technology.

  • The specificity and sensitivity of Ig subclass antibody detection were also compared.

Abstract

The outbreak of Coronavirus Disease-2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has threatened health worldwide. As of the end of 2020, there were nearly 10 million confirmed cases and nearly 5 million deaths associated with COVID-19. Rapid and early laboratory diagnosis of COVID-19 is the main focus of treatment and control. Molecular tests are the basis for confirmation of COVID-19, but serological tests for SARS-CoV-2 are widely available and play an increasingly important role in understanding the epidemiology of the virus and in identifying populations at higher risk for infection. Point-of-care tests have the advantage of rapid, accurate, portable, low cost and non-specific device requirements, which provide great help for disease diagnosis and detection. This review will discuss the performance of different laboratory diagnostic tests and platforms, as well as suitable clinical samples for testing, and related biosafety protection. This review shall guide for the diagnosis of COVID-19 caused by SARS-CoV-2.

Keywords

CoVs
SARS-CoV-2
COVID-19
nCoV-19
Laboratory diagnosis

Cited by (0)

1

Chenxi Li, Chengxue Zhao and Jingfeng Bao contributed equally to this manuscript.

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