Abstract
Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.
MeSH terms
- Animals
- Antibodies, Monoclonal / biosynthesis
- Antibodies, Monoclonal / immunology*
- Antibodies, Viral / biosynthesis
- Antibodies, Viral / immunology*
- Antibody Specificity*
- Cell Line
- Chlorocebus aethiops
- Coronavirus M Proteins
- Coronavirus Nucleocapsid Proteins
- Epitope Mapping
- Humans
- Membrane Glycoproteins / immunology
- Neutralization Tests
- Nucleocapsid Proteins / immunology
- Severe Acute Respiratory Syndrome / virology
- Severe acute respiratory syndrome-related coronavirus / immunology*
- Spike Glycoprotein, Coronavirus
- Vero Cells
- Viral Envelope Proteins / immunology
- Viral Matrix Proteins / immunology
- Viral Structural Proteins / immunology*
- Viroporin Proteins
Substances
- Antibodies, Monoclonal
- Antibodies, Viral
- Coronavirus M Proteins
- Coronavirus Nucleocapsid Proteins
- E protein, SARS coronavirus
- M protein, SARS-CoV
- Membrane Glycoproteins
- Nucleocapsid Proteins
- Spike Glycoprotein, Coronavirus
- Viral Envelope Proteins
- Viral Matrix Proteins
- Viral Structural Proteins
- Viroporin Proteins
- spike glycoprotein, SARS-CoV
- spike protein, mouse hepatitis virus