Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins

J Virol Methods. 2005 Sep;128(1-2):21-8. doi: 10.1016/j.jviromet.2005.03.021.

Abstract

Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Viral / biosynthesis
  • Antibodies, Viral / immunology*
  • Antibody Specificity*
  • Cell Line
  • Chlorocebus aethiops
  • Coronavirus M Proteins
  • Coronavirus Nucleocapsid Proteins
  • Epitope Mapping
  • Humans
  • Membrane Glycoproteins / immunology
  • Neutralization Tests
  • Nucleocapsid Proteins / immunology
  • Severe Acute Respiratory Syndrome / virology
  • Severe acute respiratory syndrome-related coronavirus / immunology*
  • Spike Glycoprotein, Coronavirus
  • Vero Cells
  • Viral Envelope Proteins / immunology
  • Viral Matrix Proteins / immunology
  • Viral Structural Proteins / immunology*
  • Viroporin Proteins

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Coronavirus M Proteins
  • Coronavirus Nucleocapsid Proteins
  • E protein, SARS coronavirus
  • M protein, SARS-CoV
  • Membrane Glycoproteins
  • Nucleocapsid Proteins
  • Spike Glycoprotein, Coronavirus
  • Viral Envelope Proteins
  • Viral Matrix Proteins
  • Viral Structural Proteins
  • Viroporin Proteins
  • spike glycoprotein, SARS-CoV
  • spike protein, mouse hepatitis virus