Abstract
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
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Project (No. 03C26213300586) supported by the National Innovation Fund for Technology, Ministry of Science and Technology of China
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Gong, Xg., Ji, J., Xie, J. et al. Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system. J. Zhejiang Univ. - Sci. B 7, 13–19 (2006). https://doi.org/10.1631/jzus.2006.B0013
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DOI: https://doi.org/10.1631/jzus.2006.B0013