Evolving technology/basic science: Thoracic
Hydrogen ameliorates pulmonary hypertension in rats by anti-inflammatory and antioxidant effects

https://doi.org/10.1016/j.jtcvs.2015.05.052 Get rights and content
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Abstract

Objective

The pathogenesis of pulmonary arterial hypertension (PAH) involves reactive oxygen species and inflammation. Beneficial effects of molecular hydrogen, which exerts both anti-inflammatory and antioxidative effects, have been reported for various pathologic conditions. We therefore hypothesized that molecular hydrogen would improve monocrotaline (MCT)-induced PAH in rats.

Methods

Nineteen male Sprague-Dawley rats (body weight: 200-300 g) were divided into groups, receiving: (1) MCT + hydrogen-saturated water (group H); (2) MCT + dehydrogenized water (group M); or (3) saline + dehydrogenized water (group C). Sixteen days after substance administration, we evaluated hemodynamics, harvested the lungs and heart, and performed morphometric analysis of the pulmonary vasculature. Macrophage infiltration, antiproliferating cell nuclear antigen–positive cells, 8-hydroxy-deoxyguanosine (8-OHdG)–positive cells, and expressions of phosphorylated signal transducers and activators of transcription-3 (STAT3) and nuclear factor of activated T-cells (NFAT) were evaluated immunohistochemically. Stromal cell–derived factor-1 and monocyte chemoattractant protein-1 expressions were evaluated by quantitative reverse-transcription polymerase chain reaction.

Results

Pulmonary arterial hypertension was significantly exacerbated in group M compared to group C, but was significantly improved in group H. Vascular density was significantly reduced in group M, but not in group H. Adventitial macrophages, antiproliferating cell nuclear antigen - and 8-OHdG–positive cells, and stromal cell–derived factor-1 and monocyte chemoattractant protein-1 expressions were significantly increased in group M, but improved in group H. Expressions of phosphorylated STAT3 and NFAT were up-regulated in group M, but improved in group H.

Conclusions

Molecular hydrogen ameliorates MCT-induced PAH in rats by suppressing macrophage accumulation, reducing oxidative stress and modulating the STAT3/NFAT axis.

CTSNet classification

9
29

Key Words

pulmonary arterial hypertension
molecular hydrogen
NFAT
STAT3
reactive oxygen species

Abbreviations and Acronyms

MCT
monocrotaline
NFAT
nuclear factor of activated T-cells
PAH
pulmonary arterial hypertension
STAT3
signal transducers and activators of transcription-3
TGFBR
transforming growth factor beta receptor
8-OHdG
8-hydroxy-deoxyguanosine

Cited by (0)

This work was supported by JSPS KAKENHI Grant Number 21790981.