Trauma/Critical Care
Hydrogen inhalation protects against acute lung injury induced by hemorrhagic shock and resuscitation
Section snippets
Animals
Male Sprague-Dawley rats weighing 300–500 g (6–9 weeks old) were purchased from Clea Japan, Inc. (Tokyo, Japan) and were kept in individual stainless steel cages in a temperature, humidity, and light-controlled room (23 ± 3°C; 55 ± 15%; 12-hour light–dark cycle) for 2–5 weeks before the experiments. During this period, all animals were provided standard food (AIN-93G diet; Oriental Kobo Corporation, Tokyo, Japan) and free access to water. All procedures involving rats were conducted in
Hydrogen improved tissue oxygenation and reduced oxidative damage
HS/R impaired pulmonary function and resulted in a remarkable decrease in Po2 levels 3 hours after resuscitation (Fig 2, A). Treatment with hydrogen after HS/R improved blood oxygenation (Fig 2, A). Neither HS/R nor hydrogen inhalation altered Pco2 levels (Fig 2, B), and there was no difference in vital signs, including blood pressure and heart rate, between the groups (data not shown). Blood lactate levels increased notably after HS/R, and this increase was suppressed significantly in the rats
Discussion
In the present study, we demonstrated that inhalation of 1.3% hydrogen significantly reduced lung injury after HS/R. Additionally, hydrogen administration did not affect the hemodynamic status during HS/R. Adjunctive therapy with inhaled hydrogen is promising and might be reasonable for lung disease as hydrogen can be easily delivered through the ventilation circuit and, thus, is straightforward therapeutic option.6, 7, 8, 16
In this study, we demonstrate an application of inhaled hydrogen
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Cited by (37)
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Hydrogen inhalation attenuates lung contusion after blunt chest trauma in mice
2023, Surgery (United States) -
Mechanism of hydrogen protection on high intensity sports injury in rats through antioxidation and its improvement of intestinal flora function
2023, Science and SportsCitation Excerpt :When the speed was 28 m/min and the time was 50 min, the rats were trained to the end of 4 weeks, 5 d per week. The method of hydrogen treatment referred to Xie [16] and Kohama [17] et al. Hydrogen was supplied through the QL-H3007 hydrogen generator (Success Hydrogen Energy Limited Company, Shandong, China), and delivered by air into the chamber through a tube.
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Co-administration of hydrogen and metformin exerts cardioprotective effects by inhibiting pyroptosis and fibrosis in diabetic cardiomyopathy
2022, Free Radical Biology and MedicineCitation Excerpt :Hydrogen has been proven to have anti-inflammatory, anti-oxidative, and anti-fibrotic properties in recent years [14,15]. Growing evidence has showed that hydrogen is effective in ischemic heart disease [16], ischemia-reperfusion injury of the small intestine and lung [17,18], and hemorrhagic shock [19]. We have previously demonstrated that inhalation of 2% hydrogen can protect against myocardial ischemia-reperfusion injury by decreasing pyroptosis [20].
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Hyperoxygenated hydrogen–rich solution suppresses shock- and resuscitation-induced liver injury
2017, Journal of Surgical ResearchCitation Excerpt :Hemorrhagic shock resulting from massive loss of blood is one of the most common complications associated with trauma or with gynecological diseases involving bleeding, peptic ulcer bleeding, and esophageal varicose vein rupture.1
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Early resuscitation with exendin-4 alleviates acute lung injury after hemorrhagic shock in rats
2017, Journal of Surgical ResearchCitation Excerpt :The morbidity and mortality associated with HS are composite results of systemic inflammatory response syndrome, which subsequently triggers multiple organ failure.3 As a major component of multiple organ failure,4 acute lung injury is a common complication after severe HS and largely accounts for the late mortality.5 Reperfusion after HS (HS/R) induces the formation of reactive oxygen species (ROS).
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Supported by JSPS KAKENHI Grant Number #25462840.