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Aging is an organ-specific process: changes in homeostasis of iron and redox proteins in the rat

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Abstract

Organ-specific changes of iron- and redox-related proteins occur with age in the rat. Ferritin, the major iron storage and detoxifying protein, as well as the proteins of the methionine-centered redox cycle (MCRC) were examined in old and young animals, and showed organ-dependent changes. In spleens and livers of aged rats, ferritin (protein) levels were greater than in young ones, and their iron saturation increased, rendering higher ferritin-bound iron (FtBI). Iron saturation of the ferritin molecule in the tongues and sternohyoids of old rats was lower but ferritin level was higher than in young rats, resulting in increased FtBI with age. Ferritin level in the esophagus of older rats was lower than in young rats but its molecular iron content higher thus the total FtBI remained the same. In the larynx, both ferritin and its iron content were the same in young and old animals. MCRC proteins were measured in livers and spleens only. With aging, methionine sulfoxide reductase A and B (MsrA and MsrB) levels in livers and spleens decreased. Thioredoxin1 (Trx) and Trx-reductase1 were elevated in old spleens, but reduced in livers. Aged spleens showed reduced Msr isozyme activity; but in the liver, its activity increased. mRNA changes with age were monitored and found to be organ specific. These organ-specific changes could reflect the different challenges and the selective pathways of each organ and its resultant capacity to cope with aging.

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Abbreviations

Ft:

Ferritin

Ft-H and Ft:

L—the H and L subunits of Ft

FtBI:

Ft-bound iron

MCRC:

Methionine-centered redox cycle

Met:

Methionine

MetO:

Methionine sulfoxide

MetO2 :

Methionine sulfone

Msr:

Methionine sulfoxide reductase

ROS:

Reactive oxygen-derived species

Trx:

Thioredoxin 1

TrxR:

Thioredoxin reductase 1

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Acknowledgments

We are grateful to Dr. A. Reznick, of the Technion-Israel Institute of Technology, for the supply the old animals. We also thank Dr. Sue Goo Rhee (Ewha Womans University, Seoul, Korea) and Dr. Jackob Moskovitz (School of Pharmacy, University of Kansas) for generously providing antibodies.

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Authors and Affiliations

  1. Department of Cellular Biochemistry and Human Genetics, The Hebrew University of Jerusalem, Faculty of Medicine and Dental Medicine, Jerusalem, Israel, 91120

    Baruch E. Bulvik, Eduard Berenshtein, Leonid Grinberg & Mordechai (Mottie) Chevion

  2. Department of Human Nutrition and Metabolism, The Hebrew University of Jerusalem, Faculty of Medicine, Jerusalem, Israel, 91120

    Abraham Marim Konijn

  3. Department of Otolaryngology/Head and Neck Surgery, The Hadassah-Hebrew University Hospital, Jerusalem, Israel

    Vladimir Vinokur & Ron Eliashar

  4. The Hebrew University of Jerusalem, The Hebrew University-Hadassah Dental and Medical Schools, P. O. B. 12272, Jerusalem, 91120, Israel

    Mordechai (Mottie) Chevion

Authors
  1. Baruch E. Bulvik
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  2. Eduard Berenshtein
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  3. Abraham Marim Konijn
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  4. Leonid Grinberg
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  5. Vladimir Vinokur
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  7. Mordechai (Mottie) Chevion
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Correspondence to Mordechai (Mottie) Chevion.

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Bulvik, B.E., Berenshtein, E., Konijn, A.M. et al. Aging is an organ-specific process: changes in homeostasis of iron and redox proteins in the rat. AGE 34, 693–704 (2012). https://doi.org/10.1007/s11357-011-9268-7

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  • DOI: https://doi.org/10.1007/s11357-011-9268-7

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