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Assessment of Spirulina-PCL nanofiber for the regeneration of dermal fibroblast layers

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Abstract

Skin is a barrier which protects injured tissues, and thus, skin regeneration is one of many important medical issues. Tissue engineering is an attractive approach to make artificial tissue or regenerate lost tissues. While constituting artificial tissues, cells must infiltrate through scaffolds, maintaining viability and proliferation. However, a three-dimensional tissue culture involves stressful environments due to several reasons such as mass or gas transport and high cell density. Once stressed, cells produce reactive oxygen species, resulting in alleviating cellular viability and activity. Spirulina is well known to have antioxidant molecules, which have been known to modulate oxidative stress to cells. Electrospun nanofiber has widely been used as a scaffold to mimic natural extracellular matrix. In this research, we assessed Spirulina extract-imbedded nanofiber as a scaffold for an artificial skin tissue. Spirulina extract was proven to positively affect viability and proliferation of mouse fibroblasts. In addition, fibroblasts infiltrated through Spirulina extract-imbedded electrospun nanofiber without cytotoxicity.

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Acknowledgments

This research was supported by grants from Marine Biotechnology Program funded by the Ministry of Land, Transport and Maritime Affairs, Korea and from Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Sang-Myung Jung, Dae Seung Kim, Jung Hyeon Ju & Hwa Sung Shin

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  1. Sang-Myung Jung
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  2. Dae Seung Kim
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  3. Jung Hyeon Ju
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  4. Hwa Sung Shin
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Correspondence to Hwa Sung Shin.

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Editor: T. Okamoto

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Jung, SM., Kim, D.S., Ju, J.H. et al. Assessment of Spirulina-PCL nanofiber for the regeneration of dermal fibroblast layers. In Vitro Cell.Dev.Biol.-Animal 49, 27–33 (2013). https://doi.org/10.1007/s11626-012-9568-y

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