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V79 Fibroblasts Are Protected Against Reactive Oxygen Species by Flax Fabric

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Abstract

Chinese hamster pulmonary fibroblasts (V79 cells) pre-treated with flax fabrics derived from non-modified or genetically engineered flax fibres and treated with H2O2 revealed a markedly lower level of intracellular reactive oxygen species (ROS) than control, non-pre-treated cells. The fabrics were prepared from fibres derived from two kinds of transgenic plants: W92 plants, which overproduce flavonoids, and M type plants, which produce hydroxybutyrate polymer in their vascular bundles and thus in fibres. Incubating the V79 cells with the flax fabrics prior to H2O2 treatment also reduced the amount of DNA damage, as established using the comet assay (also known as alkaline single-cell gel electrophoresis) and pulsed-field electrophoresis of intact cellular DNA. Selected gene expression analysis revealed the activator impact of fabrics on the apoptotic (BCL2 family, caspases) gene expression. This promoting activity was also detected for histone acetyltransferase (HAT; MYST2) gene expression. The flax fabric derived from both GM flax plants exhibited a protective effect against oxidative stress and ROS-mediated genotoxic damage, but the W92 fabric was the strongest. It is thus suggested that these fabrics might be useful as a basis for new biomedical products (e.g. wound dressings) that actively protect cells against inflammation and degeneration.

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Acknowledgements

This study was supported by grant NCBiR 178676 from the Polish Ministry of Science and Education and by Wroclaw Centre of Biotechnology, MAESTRO, from National Science Centre grant no. 2012/06/A/NZ1/00006, the Leading National Research Centre (KNOW) programme for the years 2014–2018.

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Authors and Affiliations

  1. Angiology Department, Regional Specialist Hospital in Wroclaw, Research and Development Centre, Kamienskiego 73a St, 51-124, Wroclaw, Poland

    Katarzyna Skórkowska-Telichowska

  2. Department of Basic Medical Sciences, Wroclaw Medical University, Wroclaw, Poland

    Tomasz Gębarowski, Helena Moreira, Anna Szyjka & Kazimierz Gąsiorowski

  3. Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63, 51-137, Wroclaw, Poland

    Anna Kulma, Wioleta Wojtasik, Kamil Kostyn, Aleksandra Boba, Marta Preisner, Justyna Mierziak, Malgorzata Arendt, Michał Szatkowski & Jan Szopa

  4. Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

    Jan Szopa

  5. Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63/77, 51-148, Wroclaw, Poland

    Anna Kostyn

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  1. Katarzyna Skórkowska-Telichowska
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Contributions

KST–coordinated the experiments within this manuscript and performed the data analysis, AKu–wrote the manuscript, TGe–did cell cultures and comet assays, WWo–did the gene expression analysis, KKo–participated in writing of the manuscript and prepared the manuscript for submission, HMo–performed the flow cytometry analysis, ASz–cell viability tests, ABo–did the metabolite analysis, MPr–did antioxidative assay, JMr–participated in the gene expression analysis, MAr–did pulse field electrophoresis, AKo–did the statistical analysis, MSz–participated in the metabolite analysis, JSz–general supervision, KGa–general idea of the publication.

All authors have approved the final article.

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Correspondence to Kamil Kostyn.

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Skórkowska-Telichowska, K., Kulma, A., Gębarowski, T. et al. V79 Fibroblasts Are Protected Against Reactive Oxygen Species by Flax Fabric. Appl Biochem Biotechnol 184, 366–385 (2018). https://doi.org/10.1007/s12010-017-2552-y

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