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Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils

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Abstract

The aim of study was to investigate the effects of extremely low frequency magnetic field (ELF-MF; 50 Hz; 0.1, 0.25 and 0.5 mT) on oxidative stress in the brain of 3- (adult) and 10-month-old (middle-aged) gerbils. Nitric oxide (NO) level, superoxide (O2 −) production, superoxide dismutase (SOD) activity, and index of lipid peroxidation (ILP) were measured in the forebrain cortex, striatum, hippocampus, and cerebellum immediately and 3 days after cessation of 7-day exposure. In all gerbils, ELF-MF significantly increased oxidative stress in all tested brain regions. This effect was correlated with the value of magnetic induction and was higher in middle-aged gerbils. Three days after cessation of exposure, the values of examined parameters were closer to control levels. In adult gerbils, the effect of ELF-MF of 0.1 mT on NO level, O2 − production and SOD activity was almost fully disappeared, and ILP was at the control level regardless of the value of magnetic induction. In middle-aged gerbils, the effect of ELF-MF was still present but to a lesser degree than those observed immediately after cessation of exposure. These findings pointed out the ability of ELF-MF to induce age- and magnetic induction-dependent modification of oxidative stress in the brain.

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Acknowledgments

This study was supported by Grant of the Ministry of Education, Science, and Technological Development of the Republic of Serbia (Grant No. 173027) and MMA Grant (VMA/06-10/B.4). The authors are grateful to Dr. Spomenko Mihajlović (Department of Geomagnetism and Aeronomy, Sector for Geodetic Works, Republic Geodetic Authority, Republic of Serbia) for providing geomagnetic activity data.

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The authors report no declarations of interest.

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Authors and Affiliations

  1. Institute for Medical Research, MMA, Belgrade, Serbia

    Vesna Selaković

  2. Laboratory for Magnetobiology and Behaviour, Department of Neurophysiology, Institute for Biological Research, University of Belgrade, Despota Stefana Blvd. 142, 11060, Belgrade, Serbia

    Snežana Rauš Balind, Zlatko Prolić & Branka Janać

  3. Department of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Belgrade, Serbia

    Lidija Radenović

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  1. Vesna Selaković
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  2. Snežana Rauš Balind
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  5. Branka Janać
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Correspondence to Branka Janać.

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Selaković, V., Rauš Balind, S., Radenović, L. et al. Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils. Cell Biochem Biophys 66, 513–521 (2013). https://doi.org/10.1007/s12013-012-9498-z

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  • DOI: https://doi.org/10.1007/s12013-012-9498-z

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