Eur. J. Entomol. 110 (3): 443-449, 2013 | DOI: 10.14411/eje.2013.059

Relationships between locomotor activity, oxidative damage and life span in males and females of the linden bug, Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)

Marcela BURICOVA1,2, Magdalena HODKOVA*,2
1 Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic
2 Institute of Entomology, Biology Centre, Academy of Sciences, Ceske Budejovice, Czech Republic

In this study we addressed a question of whether experimental manipulations that increase life span also reduce physical activity and molecular oxidative damage. We used three phenotypes of male and female Pyrrhocoris apterus that survive for different lengths of time, diapausing insects, reproductive insects and insects from which the corpus allatum, the source of juvenile hormone, was surgically removed. Protein carbonyl content of the thoracic muscles was used as an index of molecular oxidative modification. Diapause or ablation of the corpus allatum (allatectomy) was associated with an extended life span of both sexes, but only those individuals that were in diapause were less active. The carbonyl content, both relative (per protein unit) and absolute (per thorax) increased with age in reproductive insects of both sexes. However, the associations between the carbonyl content and diapause and allatectomy differed in males and females. In males, the carbonyl content was not associated with either diapause or allatectomy. There was no age-related increase in the relative and absolute carbonyl levels in diapausing females, while only the increase in the relative carbonyl level was absent in allectomized females. Overall, the results indicate that both allatectomy and diapause prolonged life span, but had different and sex-specific effects on locomotor activity and carbonyl content. Only the extension of the life span of diapausing females was correlated with both reduced locomotor activity and reduced carbonyl content.

Keywords: Heteroptera, Pyrrhocoridae, Pyrrhocoris apterus, corpus allatum, juvenile hormone, allatectomy, diapause, locomotor activity, survival, life span, oxidative stress, protein carbonyls, locomotor activity

Received: June 4, 2012; Revised: December 17, 2012; Accepted: December 17, 2012; Published: July 11, 2013  Show citation

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BURICOVA, M., & HODKOVA, M. (2013). Relationships between locomotor activity, oxidative damage and life span in males and females of the linden bug, Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). EJE, 110(3), 443-449. doi: 10.14411/eje.2013.059
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    References

    1. ADACHI H., FUJIWARA Y. & ISHII N. 1998: Effects of oxygen on protein carbonyl and aging in Caenorhabditis elegans mutants with long (age-1) and short (mev-1) life spans. - J. Gerontol. (A) 53: B240-B244 Go to original source...
    2. AGARWAL S. & SOHAL R. 1994: DNA oxidative damage and life expectancy in houseflies. - Proc. Natl. Acad. Sci. USA 91: 12332-12335 Go to original source...
    3. BARTKE A. 2001: Mutations prolong life in flies; implications for aging in mammals. - Trends Endocrinol. Metab. 12: 233-234 Go to original source...
    4. BLAZKOVA H., PROVAZNIK J. & HODKOVA M. 2011: Novel roles for the corpus allatum hormone in the cost of sexual interactions in the linden bug Pyrrhocoris apterus. - J. Insect Physiol. 57: 529-535 Go to original source...
    5. BROUGHTON S.J., PIPER M.D.W., IKEYA T., BASS T.M., JACOBSON J., DRIEGE Y., MARTINEZ P., HAFEN E., WITHERS D.J., LEEVERS S.J. & PARTRIDGE L. 2005: Longer lifespan, altered metabolism and stress resistance in Drosophila from ablation of cells making insulin-like ligands. - Proc. Natl. Acad. Sci. USA 102: 3105-3110 Go to original source...
    6. DAS N., LEVINE R.L., ORR W.C. & SOHAL R.S. 2001: Selectivity of protein oxidative aging in Drosophila melanogaster. - Biochem. J. 360: 209-216 Go to original source...
    7. GOTO S., NAKAMURA A., RADAK Z., NAKAMOTO H., TAKAHASHI R., YASUDA K., SKURAI Y. & ISHII N. 1999: Carbonylated proteins in aging and exercise: immunoblot approaches. - Mech. Ageing Dev. 107: 345-253 Go to original source...
    8. HARISSON J.F. & ROBERTS S.P. 2000: Flight respiration and energetics. - Annu. Rev. Physiol. 62: 179-205 Go to original source...
    9. HARMAN D. 1981: The aging process. - Proc. Natl. Acad. Sci. USA 78: 7124-7128 Go to original source...
    10. HODKOVA M. 2008: Tissue signaling pathways in the regulation of life-span and reproduction in females of the linden bug, Pyrrhocoris apterus. - J. Insect Physiol. 54: 508-517 Go to original source...
    11. HODKOVA M., BERKOVA P. & ZAHRADNICKOVA H. 2002: Photoperiodic regulation of the phospholipid molecular species composition in thoracic muscles and fat body of Pyrrhocoris apterus (Heteroptera) via an endocrine gland, corpus allatum. - J. Insect Physiol. 48: 1009-1019 Go to original source...
    12. HOLZENBERGER M., DUPONT J., DUCOS B., LENEUVE P., GELOEN A., EVEN P.C., CERVERA P. & LE BOUC Y. 2003: IGF-1 receptor regulates lifespan and resistance to oxidative stress in mice. - Nature 421: 182-187 Go to original source...
    13. HULBERT A.J., CLANCY D.J., MAIR W., BRAECKMAN B.P., GEMS D. & PARTRIDGE L. 2004: Metabolic rate is not reduced by dietary restriction or by lowered insulin/IGF-1 signalling and is not correlated with individual lifespan in Drosophila melanogaster. - Exp. Gerontol. 39: 1137-1143 Go to original source...
    14. KREGEL K.C. & ZHANG H.J. 2007: An integrated view of oxidative stress in aging: basic mechanisms, functional effects, and pathological considerations. - Am. J. Physiol. Regul. Integr. Comp. Physiol. 292: R18-R36 Go to original source...
    15. KRISHNAN N., VECERA J., KODRIK D. & SEHNAL F. 2007: 20hydroxyecdysone prevents oxidative stress damage in adult Pyrrhocoris apterus. - Arch. Insect Biochem. Physiol 65: 114-124 Go to original source...
    16. LEVINE R.L. & STADTMAN E.R. 2001: Oxidative modification of proteins during aging. - Exp. Gerontol. 36: 1495-1502 Go to original source...
    17. LEVINE R.L., GARLAND D., OLIVER C.N., AMICI A., CLIMENT I. & LENZ A.G. 1990: Determination of carbonyl content in oxidatively modified proteins. - Methods Enzymol. 186: 464-478 Go to original source...
    18. LIN K., HSIN H., LIBINA N. & KENYON C. 2001: Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling. - Nat. Genet. 28: 139-145 Go to original source...
    19. LINTON S., DAVIES M.J. & DEAN R.T. 2001: Protein oxidation and ageing. - Exp. Gerontol. 36: 1503-1518 Go to original source...
    20. LORENZ M.W. 2007: Oogenesis-flight syndrome in crickets: Age-dependent egg production, flight performance, and biochemical composition of the flight muscles in adult female Gryllus bimaculatus. - J. Insect Physiol. 53: 819-832 Go to original source...
    21. MAGWERE T., PAMPLONA R., MIWA S., MARTINEZ-DIAZ P., PORTERO-OTIN M., BRAND M.D. & PARTRIDGE L. 2006: Flight activity, mortality rates, and lipoxidative damage in Drosophila. - J. Gerontol. (A) 61: 136-145 Go to original source...
    22. MOLE S. & ZERA A.J. 1993: Differential allocation of resources underlies the dispersal-reproduction trade-off in the wingdimorphic cricket, Gryllus rubens. - Oecologia 93: 121-127 Go to original source...
    23. ORR W.C. & SOHAL R.S. 1994: Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster. - Science 263: 1128-1130 Go to original source...
    24. PEARL R. 1928: The Rate of Living. University of London Press, London, 185 pp
    25. PEREZ V.I., BOKOV A., VAN REMMEN H., MELE J., RAN Q., IKENO Y. & RICHARDSON A. 2009: Is the oxidative stress theory of aging dead? - Biochim. Biophys. Acta 1790: 1005-1014 Go to original source...
    26. PROMISLOW D.E.L. & HASELKORN T.S. 2002: Age-specific metabolic rates and mortality rates in the genus Drosophila. - Aging Cell 1: 66-74. Go to original source...
    27. REZNICK A.Z. & PACKER L. 1994: Oxidative damage to proteins - spectrometric method for carbonyl assay. - Methods Enzymol. 233: 357-363 Go to original source...
    28. SHMOOKLER REIS R.J., XU L., LEE H., CHAE M., THADEN J.J., BHARILL P., TAZEARSLAN C., SIEGEL E., ALLA R., ZIMNIAK P. & AYYADEVARA S. 2011: Modulation of lipid biosynthesis contributes to stress resistance and longevity of C. elegans mutants. - Aging 3: 125-146 Go to original source...
    29. SIM C. & DENLINGER D.L. 2008: Insulin signaling and FOXO regulate the overwintering diapause of the mosquito Culex pipiens. - Proc. Natl. Acad. Sci. USA 105: 6777-6781 Go to original source...
    30. SIM C. & DENLINGER D.L. 2009a: A shut-down in expression of an insulin-like peptide, ILP-1, halts ovarian maturation during the overwintering diapause of the mosquito Culex pipiens. - Insect Mol. Biol. 18: 325-332 Go to original source...
    31. SIM C. & DENLINGER D.L. 2009b: Transcription profiling and regulation of fat metabolism genes in diapausing adults of the mosquito Culex pipiens. - Physiol. Genomics 39: 202-209 Go to original source...
    32. SIM C. & DENLINGER D.L. 2011: Catalase and superoxide dismutase-2 enhance survival and protect ovaries during overwintering diapause in the mosquito Culex pipiens. - J. Insect Physiol. 57: 628-634 Go to original source...
    33. SLAMA K. 1964a: Hormonal control of respiratory metabolism during growth, reproduction, and diapause in male adults of Pyrrhocoris apterus L. (Hemiptera). - Biol. Bull. 127: 499-510 Go to original source...
    34. SLAMA K. 1964b: Hormonal control of respiratory metabolism during growth, reproduction, and diapause in female adults of Pyrrhocoris apterus L. (Hemiptera). - J. Insect Physiol. 10: 283-303 Go to original source...
    35. SOCHA R. & SULA J. 2006: Flight muscle polymorphism in a flightless bug, Pyrrhocoris apterus (L.): developmental pattern, biochemical profile and endocrine control. - J. Insect Physiol. 52: 231-239 Go to original source...
    36. SOCHA R. & SULA J. 2008: Regulation of the development of flight muscles in long-winged adults of the flightless bug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae). - Eur. J. Entomol. 105: 575-583 Go to original source...
    37. SOHAL R.S., AGARWAL S., DUBEY A. & ORR W.C. 1993: Protein oxidative damage is associated with life expectancy of houseflies. - Proc. Natl. Acad. Sci. USA 90: 7255-7259 Go to original source...
    38. SOHAL R.S., MOCKETT R.J. & ORR W.C. 2002: Mechanisms of aging: An appraisal of the oxidative stress hypothesis. - Free Radic. Biol. Med. 33: 575-586 Go to original source...
    39. SPEAKMAN J.R. 2005: Body size, energy metabolism and lifespan. - J. Exp. Biol. 208: 1717-1730 Go to original source...
    40. STJERNHOLM F., KARLSON B. & BOGGS C.L. 2005: Age-related changes in thoracic mass: possible reallocation of resources to reproduction in butterflies. - Biol. J. Linn. Soc. 86: 363-380 Go to original source...
    41. TANAKA S. 1993: Allocation of resources to egg production and flight muscle development in a wing dimorphic cricket, Modicogryllus confirmatus. - J. Insect Physiol. 39: 493-498 Go to original source...
    42. TATAR M., KOPELMAN A., EPSTEIN D., TU M.P., YIN C.M., CAROFALO R.S. 2001: A mutant Drosophila insulin receptor homolog that extends life-span and impairs neuroendocrine function. - Science 292: 107-110 Go to original source...
    43. VAN VORHIES W.A., KHAZAELI A.A. & CURTSINGER J.W. 2004: Testing the "rate of living" model: further evidence that longevity and metabolic rate are not inversely correlated in Drosophila melanogaster. - J. Appl. Physiol. 97: 1915-1922 Go to original source...
    44. WEGENER G. 1996: Flying insects: model systems in exercise physiology. - Experientia 52: 404-412 Go to original source...
    45. YAN L.J. & SOHAL R. 2000: Prevention of flight activity prolongs the life span of the housefly, Musca domestica, and attenuates the age-associated oxidative damage to specific mitochondrial proteins. - Free Radic. Biol. Med. 29: 1143-1150 Go to original source...

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